Abstract
A mutant of Staphylococcus aureus H was isolated by virtue of its inability to agglutinate with antibodies against teichoic acid of S. aureus. Immunological studies revealed that the mutant, S. aureus T, possessed a new surface antigen in addition to having the antigenic determinant of the wild-type strain, the ribitol teichoic acid. The presence of this additional surface component rendered strain T resistant to staphylococcal typing phages, presumably by masking the phage-receptor sites. The polymer was separated from teichoic acid by chromatography on diethylaminoethyl cellulose and was shown to be composed of two amino sugars, N-acetyl-d-fucosamine and N-acetyl-d-mannosamin uronic acid.
Full text
PDF










Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ashwell G., Brown N. C., Volk W. A. A colorimetric procedure for the determination of N-acetylated-3-amino hexoses. Arch Biochem Biophys. 1965 Dec;112(3):648–652. doi: 10.1016/0003-9861(65)90108-6. [DOI] [PubMed] [Google Scholar]
- BARKER S. A., BRIMACOMBE J. S., HOW M. J., STACEY M., WILLIAMS J. M. Two new amino-sugars from an antigenic polysaccharide of Pneumococcus. Nature. 1961 Jan 28;189:303–304. doi: 10.1038/189303a0. [DOI] [PubMed] [Google Scholar]
- BARRY G. T., ROARK E. L-FUCOSAMINE AND 4-OXO-NORLEUCINE AS CONSTITUENTS IN MUCOPOLYSACCHARIDES OF CERTAIN ENTERIC BACTERIA. Nature. 1964 May 2;202:493–494. doi: 10.1038/202493a0. [DOI] [PubMed] [Google Scholar]
- CRUMPTON M. J., DAVIES D. A. The isolation of D-fucosamine from the specific polysaccharide of Chromobacterium violaceum (NCTC 7917). Biochem J. 1958 Dec;70(4):729–736. doi: 10.1042/bj0700729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chatterjee A. N., Mirelman D., Singer H. J., Park J. T. Properties of a novel pleiotropic bacteriophage-resistant mutant of Staphylococcus aureus H. J Bacteriol. 1969 Nov;100(2):846–853. doi: 10.1128/jb.100.2.846-853.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chatterjee A. N. Use of bacteriophage-resistant mutants to study the nature of the bacteriophage receptor site of Staphylococcus aureus. J Bacteriol. 1969 May;98(2):519–527. doi: 10.1128/jb.98.2.519-527.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coyette J., Ghuysen J. M. Structure of the cell wall of Staphylococcus aureus, strain Copenhagen. IX. Teichoic acid and phage adsorption. Biochemistry. 1968 Jun;7(6):2385–2389. doi: 10.1021/bi00846a048. [DOI] [PubMed] [Google Scholar]
- Ellwood D. C., Tempest D. W. Control of teichoic acid and teichuronic acid biosyntheses in chemostat cultures of Bacillus subtilis var. niger. Biochem J. 1969 Jan;111(1):1–5. doi: 10.1042/bj1110001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ghuysen J. M., Bricas E., Leyh-Bouille M., Lache M., Shockman G. D. The peptide N alpha-(L-alanyl-D-isoglutaminyl)-N epsilon-(D-isoasparaginyl)-L-lysyl-D-alanine and the disaccharide N-acetylglucosaminyl-beta-1,4-N-acetylmuramic acid in cell wall peptidoglycan of Streptococcus faecalis strain ATCC 9790. Biochemistry. 1967 Aug;6(8):2607–2619. doi: 10.1021/bi00860a044. [DOI] [PubMed] [Google Scholar]
- Hase S., Matsushima Y. Structural studies on a glucose-containing polysaccharide obtained from Micrococcus lysodeikticus cell walls. II. Determination of the linkage points on N-acetyl-mannosaminuronic acid and its configuration. J Biochem. 1971 Mar;69(3):559–565. [PubMed] [Google Scholar]
- Heptinstall S., Archibald A. R., Baddiley J. Teichoic acids and membrane function in bacteria. Nature. 1970 Feb 7;225(5232):519–521. doi: 10.1038/225519a0. [DOI] [PubMed] [Google Scholar]
- JUERGENS W. G., SANDERSON A. R., STROMINGER J. L. Chemical basis for an immunological specificity of a strain of Staphylococcus aureus. J Exp Med. 1963 Jun 1;117:925–935. doi: 10.1084/jem.117.6.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIN E. C., LERNER S. A., JORGENSEN S. E. A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes. Biochim Biophys Acta. 1962 Jul 2;60:422–424. doi: 10.1016/0006-3002(62)90423-7. [DOI] [PubMed] [Google Scholar]
- LUDOWIEG J., DORFMAN A. A micromethod for the colorimetric determination of N-acetyl groups in acid mucopolysaccharides. Biochim Biophys Acta. 1960 Feb 26;38:212–218. doi: 10.1016/0006-3002(60)91233-6. [DOI] [PubMed] [Google Scholar]
- PERKINS H. R. A polymer containing glucose and aminohexuronic acid isolated from the cell walls of micrococcus lysodeikticus. Biochem J. 1963 Mar;86:475–483. doi: 10.1042/bj0860475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PLESCIA O. J., BRAUN W., PALCZUK N. C. PRODUCTION OF ANTIBODIES TO DENATURED DEOXYRIBONUCLEIC ACID (DNA). Proc Natl Acad Sci U S A. 1964 Aug;52:279–285. doi: 10.1073/pnas.52.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REISSIG J. L., STORMINGER J. L., LELOIR L. F. A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem. 1955 Dec;217(2):959–966. [PubMed] [Google Scholar]
- RONDLE C. J., MORGAN W. T. The determination of glucosamine and galactosamine. Biochem J. 1955 Dec;61(4):586–589. doi: 10.1042/bj0610586. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SPIRO R. G. Studies on the biosynthesis of glucosamine in the intact rat. J Biol Chem. 1959 Apr;234(4):742–748. [PubMed] [Google Scholar]
- STOFFYN P. J., JEANLOZ R. W. Identification of amino sugars by paper chromatography. Arch Biochem Biophys. 1954 Oct;52(2):373–379. doi: 10.1016/0003-9861(54)90137-x. [DOI] [PubMed] [Google Scholar]
- Sharon N., Shif I., Zehavi U. The isolation of D-fucosamine (2-amino-2,6-dideoxy-D-galactose) from polysaccharides of Bacillus. Biochem J. 1964 Oct;93(1):210–214. doi: 10.1042/bj0930210. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw D. R., Mirelman D., Chatterjee A. N., Park J. T. Ribitol teichoic acid synthesis in bacteriophage-resistant mutants of Staphylococcus aureus H. J Biol Chem. 1970 Oct 10;245(19):5101–5106. [PubMed] [Google Scholar]
- WENTWORTH B. B. BACTERIOPHAGE TYPING OF THE STAPHYLOCOCCI. Bacteriol Rev. 1963 Sep;27:253–272. doi: 10.1128/br.27.3.253-272.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHEAT R., ROLLINS E. L., LEATHERWOOD J. ISOLATION OF D-FUCOSAMINE FROM BACILLUS CEREUS. Nature. 1964 May 2;202:492–493. doi: 10.1038/202492a0. [DOI] [PubMed] [Google Scholar]
- WILEY B. B. A new virulence test for Staphylococcus aureus and its application to encapsulated strains. Can J Microbiol. 1961 Dec;7:933–943. doi: 10.1139/m61-118. [DOI] [PubMed] [Google Scholar]
- WILKINSON J. F. The extracellualr polysaccharides of bacteria. Bacteriol Rev. 1958 Mar;22(1):46–73. doi: 10.1128/br.22.1.46-73.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]