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. 1971 Dec;108(3):1135–1140. doi: 10.1128/jb.108.3.1135-1140.1971

Biosynthesis of 7,8-Diaminopelargonic Acid, a Biotin Intermediate, from 7-Keto-8-Aminopelargonic Acid and S-Adenosyl-l-Methionine1

Max A Eisenberg a, Gerald L Stoner a,2
PMCID: PMC247197  PMID: 4945185

Abstract

Resting cells of Escherichia coli strain D302(bioD302) can synthesize 7,8-diaminopelargonic acid from 7-keto-8-aminopelargonic acid. The product of this aminotransferase reaction has been identified by paper chromatography and electrophoresis. Glucose enhances the vitamer yield twofold. Of the 19 amino acids tested as amino donors, only methionine proved to be significantly stimulatory. In cell-free extracts, however, methionine was completely inactive unless both adenosine triphosphate (ATP) and Mg2+ were present. S-Adenosyl-l-methionine (SAM) was about 10 times more effective than methionine, ATP, and Mg2+. The optimal conditions for the reaction were determined, and substrate inhibition was found for 7-keto-8-aminopelargonic acid. It has been possible to eliminate certain impurities as amino donors in the commercial preparation of SAM and those that may arise in enzymatic reactions in which SAM is a substrate. The direct participation of SAM in the aminotransferase reaction seems a likely possibility.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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