Abstract
During the development of fruit bodies of Schizophyllum commune there is a minimum 10- to 15-fold increase in amylase activity. There is little or no activity in homokaryons or dikaryons. The activity is found early after the onset of morphogenesis and increases until the fruit bodies are mature. Inhibition studies with CO2 indicate that the activity is directly associated with fruiting, as a change from fruiting to vegetative growth of the dikaryotic mycelium leads to a loss in activity, whereas the already formed fruit bodies show no loss. The activity is unaffected by the level of glucose in the medium. Evidence is presented, based on the mode of starch degradation and on yield and inhibition studies, that the enzyme is a glucoamylase.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HSIU J., FISCHER E. H., STEIN E. A. ALPHA-AMYLASES AS CALCIUM-METALLOENZYMES. II. CALCIUM AND THE CATALYTIC ACTIVITY. Biochemistry. 1964 Jan;3:61–66. doi: 10.1021/bi00889a011. [DOI] [PubMed] [Google Scholar]
- King N. J. The extracellular enzymes of Coniophora cerebulla. Biochem J. 1966 Sep;100(3):784–792. doi: 10.1042/bj1000784. [DOI] [PMC free article] [PubMed] [Google Scholar]
- King N. J. The glucoamylase of Coniophora cerebella. Biochem J. 1967 Nov;105(2):577–583. doi: 10.1042/bj1050577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leonard T. J., Dick S. CHEMICAL INDUCTION OF HAPLOID FRUITING BODIES IN Schizophyllum commune. Proc Natl Acad Sci U S A. 1968 Mar;59(3):745–751. doi: 10.1073/pnas.59.3.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NIEDERPRUEM D. J. ROLE OF CARBON DIOXIDE IN THE CONTROL OF FRUITING OF SCHIZOPHYLLUM COMMUNE. J Bacteriol. 1963 Jun;85:1300–1308. doi: 10.1128/jb.85.6.1300-1308.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wessels J. G. A beta 1, 6-glucan glucanohydrolase involved in hydrolysis of cell-wall glucan in Schizophyllum commune. Biochim Biophys Acta. 1969 Mar 18;178(1):191–193. doi: 10.1016/0005-2744(69)90149-1. [DOI] [PubMed] [Google Scholar]
- Wessels J. G. Control of cell-wall glucan degradation during development in Schizophyllum commune. Antonie Van Leeuwenhoek. 1966;32(4):341–355. doi: 10.1007/BF02097484. [DOI] [PubMed] [Google Scholar]
- Wessels J. G., Niederpruem D. J. Role of a cell-wall glucan-degrading enzyme in mating of Schizophyllum commune. J Bacteriol. 1967 Nov;94(5):1594–1602. doi: 10.1128/jb.94.5.1594-1602.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]