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. 1972 Jan;109(1):116–121. doi: 10.1128/jb.109.1.116-121.1972

Regulation of Penicillinase Synthesis: a Mutation in Staphylococcus aureus Unlinked to the Penicillinase Plasmid That Reduces Penicillinase Inducibility

J Weaver Zyskind 1, John Imsande 2
PMCID: PMC247258  PMID: 4333373

Abstract

A mutant of Staphylococcus aureus strain 655 was isolated that is restricted in penicillinase induction. Wild-type plasmids that bear penicillinase determinants could not be fully induced in this mutant, 655par-1; hence, the responsible mutation is not located on the plasmid. Mutant plasmid PI258penI443, which produces penicillinase constitutively in wild-type cells, was fully constitutive for penicillinase production when it was harbored by mutant 655par-1. Therefore, the bacterial mutation does not interfere directly with the transcription of the penZ gene or translation of the penicillinase messenger ribonucleic acid. Mutant plasmid PII147penI220 was fully inducible in the mutant bacterium, even though the wild-type plasmid PII147 was only partially inducible in the par-1 mutant. Thus, in the presence of inducer, complementation appears to occur between the product of the par-1 gene and the product of the penI220 gene. These results suggest that the par-1 gene codes for a penicillinase antire-pressor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berryhill D. L., Pattee P. A. Buoyant density analysis of staphylococcal bacteriophage 80 transducing particles. J Virol. 1969 Nov;4(5):804–806. doi: 10.1128/jvi.4.5.804-806.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen S., Sweeney H. M. Constitutive penicillinase formation in Staphylococcus aureus owing to a mutation unlinked to the penicillinase plasmid. J Bacteriol. 1968 Apr;95(4):1368–1374. doi: 10.1128/jb.95.4.1368-1374.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S., Vernon E. G., Sweeney H. M. Differential depression of staphylococcal plasmid and chromosomal penicillinase genes by a class of unlinked chromosomal mutations (R2-). J Bacteriol. 1970 Sep;103(3):616–621. doi: 10.1128/jb.103.3.616-621.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Collins J. F., Mandelstam J., Pollock M. R., Richmond M. H., Sneath P. H. A suggested phenotypic classification and terminology for enzyme mutants in micro-organisms. Nature. 1965 Nov 27;208(5013):841–843. doi: 10.1038/208841a0. [DOI] [PubMed] [Google Scholar]
  5. HARMON S. A., BALDWIN J. N. NATURE OF THE DETERMINANT CONTROLLING PENICILLINASE PRODUCTION IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1964 Mar;87:593–597. doi: 10.1128/jb.87.3.593-597.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Imsande J. Regulation of penicillinase synthesis: evidence for a unified model. J Bacteriol. 1970 Jan;101(1):173–180. doi: 10.1128/jb.101.1.173-180.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Imsande J., Zyskind J. W., Mile I. Regulation of staphylococcal penicillinase synthesis. J Bacteriol. 1972 Jan;109(1):122–133. doi: 10.1128/jb.109.1.122-133.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEITNER F., SWEENEY H. M., MARTIN T. F., COHEN S. INDUCTION OF STAPHYLOCOCCAL PENICILLINASE BY BENZYLPENICILLIN: EFFECT OF PH, CONCENTRATION OF FERROUS ION AND INDUCER, AND DURATION OF EXPOSURE OF CELLS TO INDUCER. J Bacteriol. 1963 Oct;86:717–727. doi: 10.1128/jb.86.4.717-727.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MAY J. W., HOUGHTON R. H., PERRET C. J. THE EFFECT OF GROWTH AT ELEVATED TEMPERATURES ON SOME HERITABLE PROPERTIES OF STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1964 Nov;37:157–169. doi: 10.1099/00221287-37-2-157. [DOI] [PubMed] [Google Scholar]
  10. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  11. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PATTEE P. A., BALDWIN J. N. Transduction of resistance to some macrolide antibiotics in Staphylococcus aureus. J Bacteriol. 1962 Nov;84:1049–1055. doi: 10.1128/jb.84.5.1049-1055.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PERRET C. J. Iodometric assay of penicillinase. Nature. 1954 Nov 27;174(4439):1012–1013. doi: 10.1038/1741012a0. [DOI] [PubMed] [Google Scholar]
  14. Peyru G., Wexler L. F., Novick R. P. Naturally occurring penicillinase plasmids in Staphylococcus aureus. J Bacteriol. 1969 Apr;98(1):215–221. doi: 10.1128/jb.98.1.215-221.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RICHMOND M. H. DOMINANCE OF THE INDUCIBLE STATE IN STRAINS OF STAPHYLOCOCCUS AUREUS CONTAINING TWO DISTINCT PENICILLINASE PLASMIDS. J Bacteriol. 1965 Aug;90:370–374. doi: 10.1128/jb.90.2.370-374.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Richmond M. H. A second regulatory region involved in penicillinase synthesis in Staphylococcus aureus. J Mol Biol. 1967 Jun 14;26(2):357–360. doi: 10.1016/0022-2836(67)90305-1. [DOI] [PubMed] [Google Scholar]

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