Abstract
Yeast mutants deficient in orotidine-5′-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, to derepress, or to induce pyrimidine biosynthesis. This enzyme appears, therefore, to escape the scheme of regulation by mixed repression and induction controlling the other enzymes of the pyrimidine pathway.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BECKWITH J. R., PARDEE A. B., AUSTRIAN R., JACOB F. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol. 1962 Dec;5:618–634. doi: 10.1016/s0022-2836(62)80090-4. [DOI] [PubMed] [Google Scholar]
- Caroline D. F. Pyrimidine synthesis in Neurospora crassa: gene-enzyme relationships. J Bacteriol. 1969 Dec;100(3):1371–1377. doi: 10.1128/jb.100.3.1371-1377.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HANDSCHUMACHER R. E. Orotidylic acid decarboxylase: inhibition studies with azauridine 5'-phosphate. J Biol Chem. 1960 Oct;235:2917–2919. [PubMed] [Google Scholar]
- HEPPEL L. A., HILMOE R. J. Purification of yeast inorganic pyrophosphatase. J Biol Chem. 1951 Sep;192(1):87–94. [PubMed] [Google Scholar]
- Jund R., Lacroute F. Genetic and physiological aspects of resistance to 5-fluoropyrimidines in Saccharomyces cerevisiae. J Bacteriol. 1970 Jun;102(3):607–615. doi: 10.1128/jb.102.3.607-615.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIEBERMAN I., KORNBERG A., SIMMS E. S. Enzymatic synthesis of pyrimidine nucleotides; orotidine-5'-phosphate and uridine-5'-phosphate. J Biol Chem. 1955 Jul;215(1):403–451. [PubMed] [Google Scholar]
- Lacroute F. Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1968 Mar;95(3):824–832. doi: 10.1128/jb.95.3.824-832.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mortimer R. K., Hawthorne D. C. Genetic mapping in Saccharomyces. Genetics. 1966 Jan;53(1):165–173. doi: 10.1093/genetics/53.1.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Donovan G. A., Neuhard J. Pyrimidine metabolism in microorganisms. Bacteriol Rev. 1970 Sep;34(3):278–343. doi: 10.1128/br.34.3.278-343.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pinsky L., Krooth R. S. Studies on the control of pyrimidine biosynthesis in human diploid cell strains, I. Effect of 6-azauridine on cellular phenotype. Proc Natl Acad Sci U S A. 1967 Apr;57(4):925–932. doi: 10.1073/pnas.57.4.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pinsky L., Krooth R. S. Studies on the control of pyrimidine biosynthesis in human diploid cell strains. II. Effects of 5-azaorotic acid, barbituric acid, and pyrimidine precursors on cellular phenotype. Proc Natl Acad Sci U S A. 1967 May;57(5):1267–1274. doi: 10.1073/pnas.57.5.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reyes P. The synthesis of 5-fluorouridine 5'-phosphate by a pyrimidine phosphoribosyltransferase of mammalian origin. I. Some properties of the enzyme from P1534J mouse leukemic cells. Biochemistry. 1969 May;8(5):2057–2062. doi: 10.1021/bi00833a041. [DOI] [PubMed] [Google Scholar]