Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Jan;109(1):276–284. doi: 10.1128/jb.109.1.276-284.1972

Biosynthesis of 50S Ribosomal Proteins During the Outgrowth of Bacillus subtilis Spores1

Marlene V Shaw a, Robert L Armstrong a
PMCID: PMC247277  PMID: 4621627

Abstract

The biosynthesis of 14 protein bands from 50S ribosomes was studied during the outgrowth of Bacillus subtilis W23 spores. The time at which the synthesis of each protein band begins and the patterns of synthesis that each displays were determined by comparing the incorporation of 3H-phenylalanine during five intervals throughout the first hour of outgrowth with the incorporation of 14C-phenylalanine between 145 and 150 min. The majority of the proteins in the 14 bands analyzed were synthesized as early as 5 to 10 min. The bands could be grouped on the basis of their labeling patterns as well as the time required for them to achieve a high rate of synthesis. Three bands did not achieve a high rate of synthesis until after 30 min of outgrowth, and at least one of the proteins from these bands appeared to be located on the exterior of the 50S subunit. The significance of the proteins from these three bands is discussed in terms of the late formation of new, mature 50S subunits during outgrowth.

Full text

PDF
276

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong R. L., Sueoka N. Phase transitions in ribonucleic acid synthesis during germination of Bacillus subtilis spores. Proc Natl Acad Sci U S A. 1968 Jan;59(1):153–160. doi: 10.1073/pnas.59.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis F. C., Jr, Sells B. H. Synthesis and assembly of ribosomal protein into 50 s subunits during recovery from chloramphenicol treatment. J Mol Biol. 1969 Feb 14;39(3):503–521. doi: 10.1016/0022-2836(69)90141-7. [DOI] [PubMed] [Google Scholar]
  3. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  4. Fogel S., Sypherd P. S. Extraction and isolation of individual ribosomal proteins from Escherichia coli. J Bacteriol. 1968 Aug;96(2):358–364. doi: 10.1128/jb.96.2.358-364.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gesteland R. F., Staehelin T. Electrophoretic analysis of proteins from normal and cesium chloride-treated Escherichia coli ribosomes. J Mol Biol. 1967 Mar 14;24(2):149–155. doi: 10.1016/0022-2836(67)90323-3. [DOI] [PubMed] [Google Scholar]
  6. Goldthwaite C., Dubnau D., Smith I. Genetic mapping of antibiotic resistance in markers Bacillus subtilis. Proc Natl Acad Sci U S A. 1970 Jan;65(1):96–103. doi: 10.1073/pnas.65.1.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gray R. H., Steffensen D. M. A high-resolution method for slicing and counting radioactivity in polyacrylamide gels. Anal Biochem. 1968 Jul;24(1):44–53. doi: 10.1016/0003-2697(68)90058-4. [DOI] [PubMed] [Google Scholar]
  8. Harford N., Sueoka N. Chromosomal location of antibiotic resistance markers in Bacillus subtilis. J Mol Biol. 1970 Jul 28;51(2):267–286. doi: 10.1016/0022-2836(70)90142-7. [DOI] [PubMed] [Google Scholar]
  9. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1276–1282. doi: 10.1073/pnas.67.3.1276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MESELSON M., NOMURA M., BRENNER S., DAVERN C., SCHLESSINGER D. CONSERVATION OF RIBOSOMES DURING BACTERIAL GROWTH. J Mol Biol. 1964 Sep;9:696–711. doi: 10.1016/s0022-2836(64)80176-5. [DOI] [PubMed] [Google Scholar]
  11. Nukushina J. I., Ikeda Y. Genetic analysis of the developmental processes during germination and outgrowth of Bacillus subtilis spores with temperature-sensitive mutants. Genetics. 1969 Sep;63(1):63–74. doi: 10.1093/genetics/63.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. O'Sullivan A., Sueoka N. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment. J Mol Biol. 1967 Jul 28;27(2):349–368. doi: 10.1016/0022-2836(67)90025-3. [DOI] [PubMed] [Google Scholar]
  13. OISHI M., YOSHIKAWA H., SUEOKA N. SYNCHRONOUS AND DICHOTOMOUS REPLICATIONS OF THE BACILLUS SUBTILIS CHROMOSOME DURING SPORE GERMINATION. Nature. 1964 Dec 12;204:1069–1073. doi: 10.1038/2041069a0. [DOI] [PubMed] [Google Scholar]
  14. Oishi M., Oishi A., Sueoka N. Location of genetic loci of soluble RNA on Bacillus subtilis chromosome. Proc Natl Acad Sci U S A. 1966 May;55(5):1095–1103. doi: 10.1073/pnas.55.5.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Oishi M., Sueoka N. Location of genetic loci of ribosomal RNA on Bacillus subtilis chromosome. Proc Natl Acad Sci U S A. 1965 Aug;54(2):483–491. doi: 10.1073/pnas.54.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith I., Dubnau D., Morrell P., Marmur J. Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis. J Mol Biol. 1968 Apr 14;33(1):123–140. doi: 10.1016/0022-2836(68)90285-4. [DOI] [PubMed] [Google Scholar]
  17. Smith I., Goldthwaite C., Dubnau D. The genetics of ribosomes in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1969;34:85–89. doi: 10.1101/sqb.1969.034.01.013. [DOI] [PubMed] [Google Scholar]
  18. Staehelin T., Maglott D. M., Monro R. E. On the catalytic center of peptidyl transfer: a part of the 50 S ribosome structure. Cold Spring Harb Symp Quant Biol. 1969;34:39–48. doi: 10.1101/sqb.1969.034.01.008. [DOI] [PubMed] [Google Scholar]
  19. Traut R. R., Delius H., Ahmad-Zadeh C., Bickle T. A., Pearson P., Tissières A. Ribosomal proteins of E. Coli: stoichiometry and implications for ribosome structure. Cold Spring Harb Symp Quant Biol. 1969;34:25–38. doi: 10.1101/sqb.1969.034.01.007. [DOI] [PubMed] [Google Scholar]
  20. WAKE R. G. SEQUENTIAL REPLICATION OF DNA IN SYNCHRONOUSLY GERMINATING BACILLUS SUBTILIS SPORES. Biochem Biophys Res Commun. 1963 Sep 10;13:67–70. doi: 10.1016/0006-291x(63)90164-5. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES