Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Jan;109(1):285–291. doi: 10.1128/jb.109.1.285-291.1972

Transfection of Staphylococcus aureus with Bacteriophage Deoxyribonucleic Acid

Jan-Eric Sjöström 1, Martin Lindberg 1, Lennart Philipson 1
PMCID: PMC247278  PMID: 4257981

Abstract

Staphylococcus aureus cells of strain 8325 (N) are competent for phage deoxyribonucleic acid (DNA) when harvested in the early exponential growth phase. Phenotypic expression of the competence requires divalent cations, and calcium ions are most effective. Treatment of phage DNA with deoxyribonuclease completely destroys infectivity and heat-denaturated DNA is not infectious. The highest frequency of transfection is around 104 plaque-forming units per μg of DNA.

Full text

PDF
285

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRODY E., COLEMAN L., MACKAL R. P., WERNINGHAUS B., EVANS E. A., Jr PROPERTIES OF INFECTIOUS DEOXYRIBONUCLEIC ACID FROM T1 AND LAMBDA BACTERIOPHAGE. J Biol Chem. 1964 Jan;239:285–289. [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARERE A., SPADA-SERMONTI I. NUTRITIONAL MUTATIONS AND TRANSDUCTION BY ULTRAVIOLET-INACTIVATED PHAGE IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1964 Jul;88:226–232. doi: 10.1128/jb.88.1.226-232.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fraser D., Mahler H. R., Shug A. L., Thomas C. A. THE INFECTION OF SUB-CELLULAR ESCHERICHIA COLI, STRAIN B, WITH A DNA PREPARATION FROM T2 BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1957 Nov 15;43(11):939–947. doi: 10.1073/pnas.43.11.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GREEN D. M. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82. J Mol Biol. 1964 Dec;10:438–451. doi: 10.1016/s0022-2836(64)80065-6. [DOI] [PubMed] [Google Scholar]
  6. GREEN M., PINA M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964 Jun;51:1251–1259. doi: 10.1073/pnas.51.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KAISER A. D., HOGNESS D. S. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg. J Mol Biol. 1960 Dec;2:392–415. doi: 10.1016/s0022-2836(60)80050-2. [DOI] [PubMed] [Google Scholar]
  8. Kloos W. E. Factors affecting transformation of Micrococcus lysodeikticus. J Bacteriol. 1969 Jun;98(3):1397–1399. doi: 10.1128/jb.98.3.1397-1399.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  10. Mandel M. Infectivity of phage P2 DNA in presence of helper phage. Mol Gen Genet. 1967;99(1):88–96. doi: 10.1007/BF00306461. [DOI] [PubMed] [Google Scholar]
  11. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  12. Okanishi M., Utahara R., Okami Y. Infection of the protoplasts of Streptomyces kanamyceticus with deoxyribonucleic acid preparation from actinophage PK-66. J Bacteriol. 1966 Dec;92(6):1850–1852. doi: 10.1128/jb.92.6.1850-1852.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Omenn G. S., Friedman J. Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity. J Bacteriol. 1970 Mar;101(3):921–924. doi: 10.1128/jb.101.3.921-924.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. REILLY B. E., SPIZIZEN J. BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS. J Bacteriol. 1965 Mar;89:782–790. doi: 10.1128/jb.89.3.782-790.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Riggs H. G., Jr, Rosenblum E. D. Transfection of lysostaphin-treated cells of Staphylococcus aureus. J Virol. 1969 Jan;3(1):33–37. doi: 10.1128/jvi.3.1.33-37.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spizizen J. INFECTION OF PROTOPLASTS BY DISRUPTED T2 VIRUS. Proc Natl Acad Sci U S A. 1957 Aug 15;43(8):694–701. doi: 10.1073/pnas.43.8.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spizizen J., Reilly B. E., Evans A. H. Microbial transformation and transfection. Annu Rev Microbiol. 1966;20:371–400. doi: 10.1146/annurev.mi.20.100166.002103. [DOI] [PubMed] [Google Scholar]
  18. Streips U. N., Welker N. E. Infection of Bacillus stearothermophilus with bacteriophage deoxyribonucleic acid. J Bacteriol. 1969 Jul;99(1):344–346. doi: 10.1128/jb.99.1.344-346.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. YOUNG F. E., SPIZIZEN J. INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM. J Bacteriol. 1963 Sep;86:392–400. doi: 10.1128/jb.86.3.392-400.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES