Abstract
Transformation-deficient (com−) mutants, which are able to bind donor transforming deoxyribonucleic acid (DNA) without yielding a significant number of transformants, were studied with regard to the fate of donor DNA. In no case was there any detectable degradation into acid-soluble radioactivity after donor DNA uptake. Physical experiments showed that some of these mutants are deficient in their ability to associate donor DNA with the recipient's chromosome (dad− mutants, for donor association defective), whereas others are able to form what appear to be normal donor-recipient complexes. In spite of physical evidence for integration, none of the dad− mutants contains biologically active recombinant DNA, suggesting that they might be deficient in the recombination process (dab− mutants, for donor association biologically defective). Donor biological activity is not replicated in any of the mutant strains, and in some cases there is a 10-fold reduction of donor transforming DNA within 60 min after DNA uptake.
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Selected References
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