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. 1972 Jan;109(1):298–306. doi: 10.1128/jb.109.1.298-306.1972

Competence Mutants III. Responses to Radiations

Edith Horn Postel 1, Sol H Goodgal 1
PMCID: PMC247280  PMID: 4550668

Abstract

Class 3 com− mutants [normal in deoxyribonucleic acid (DNA) uptake but poor in ability to transform] were investigated with regard to ultraviolet (UV) and X-ray sensitivity of colony-forming ability and with regard to their ability to be transformed by UV- and X-ray-irradiated DNA. Three mutants, com−40, 60, and 78, were highly UV-sensitive in colony-forming ability. None of the mutants was more sensitive than wild type to UV-irradiated transforming DNA; in fact, six of the mutants showed considerably greater resistance. Two of the mutants (com−40 and 60) were slightly more sensitive to X ray in colony formation, whereas most of the mutants showed some degree of sensitivity to X-ray-irradiated transforming DNA. In addition, the physical fate of X-ray-irradiated transforming DNA has been examined, and in one case (com−48) there was a significant drop in sedimentation value of X-ray-irradiated donor DNA after uptake by recipient cells. The com− mutants analyzed have been classified on the basis of their UV and X-ray sensitivities, and, where appropriate, possible biochemical lesions have been implicated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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