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. 1972 Jan;109(1):373–378. doi: 10.1128/jb.109.1.373-378.1972

Mode of Cell Wall Growth of Bacillus megaterium

J Mauck 1, L Chan 1, L Glaser 1, J Williamson 1
PMCID: PMC247287  PMID: 4621629

Abstract

The growth of the cell wall of Bacillus megaterium KM was examined by labeling the cell wall uniformly with 3H-α, ε-diaminopimelic acid and examining by radioautography the distribution of labeled cell wall material in daughter cells. The results indicate that the label is distributed uniformly to all daughter cells and rule out a single growth point, either central or apical, for these cells. The results strongly suggest that cell wall growth occurs by uniform deposition of new wall over the whole cell surface.

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Selected References

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  1. Briles E. B., Tomasz A. Radioautographic evidence for equatorial wall growth in a gram-positive bacterium. Segregation of choline-3H-labeled teichoic acid. J Cell Biol. 1970 Dec;47(3):786–790. doi: 10.1083/jcb.47.3.786. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHALOUPKA J., KRECKOVA P., RIHOVA L. The mucopeptide turnover in the cell walls of growing cultures of Bacillus megaterium KM. Experientia. 1962 Aug 15;18:362–363. doi: 10.1007/BF02172250. [DOI] [PubMed] [Google Scholar]
  3. CHUNG K. L., HAWIRKO R. Z., ISAAC P. K. CELL WALL REPLICATION. I. CELL WALL GROWTH OF BACILLUS CEREUS AND BACILLUS MEGATERIUM. Can J Microbiol. 1964 Feb;10:43–48. doi: 10.1139/m64-007. [DOI] [PubMed] [Google Scholar]
  4. Chung K. L. Autoradiographic studies of bacterial cell wall replication. I. Cell wall growth of Bacillus cereus in the presence of chloramphenicol. Can J Microbiol. 1967 Apr;13(4):341–350. doi: 10.1139/m67-046. [DOI] [PubMed] [Google Scholar]
  5. Cole R. M. Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence. Bacteriol Rev. 1965 Sep;29(3):326–344. doi: 10.1128/br.29.3.326-344.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fukuda A., Gilvarg C. The relationship of dipicolinate and lysine biosynthesis in Bacillus megaterium. J Biol Chem. 1968 Jul 25;243(14):3871–3876. [PubMed] [Google Scholar]
  7. Higgins M. L., Shockman G. D. Early changes in the ultrastructure of Streptococcus faecalis after amino acid starvation. J Bacteriol. 1970 Jul;103(1):244–253. doi: 10.1128/jb.103.1.244-253.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hughes R. C., Stokes E. Cell wall growth in Bacillus licheniformis followed by immunofluorescence with mucopeptide-specific antiserum. J Bacteriol. 1971 May;106(2):694–696. doi: 10.1128/jb.106.2.694-696.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Levy S. B., Leive L. An equilibrium between two fractions of lipopolysaccharide in Escherichia coli. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1435–1439. doi: 10.1073/pnas.61.4.1435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mauck J., Chan L., Glaser L. Turnover of the cell wall of Gram-positive bacteria. J Biol Chem. 1971 Mar 25;246(6):1820–1827. [PubMed] [Google Scholar]
  11. Mauck J., Glaser L. Turnover of the cell wall of Bacillus subtilis W-23 during logarithmic growth. Biochem Biophys Res Commun. 1970 May 22;39(4):699–706. doi: 10.1016/0006-291x(70)90261-5. [DOI] [PubMed] [Google Scholar]
  12. Mindich L. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol. 1970 Apr 28;49(2):415–432. doi: 10.1016/0022-2836(70)90254-8. [DOI] [PubMed] [Google Scholar]
  13. Morrison D. C., Morowitz H. J. Studies on membrane synthesis in Bacillus megaterium KM. J Mol Biol. 1970 Apr 28;49(2):441–459. doi: 10.1016/0022-2836(70)90256-1. [DOI] [PubMed] [Google Scholar]
  14. RYTER A., JACOB F. ETUDE AU MICROSCOPE 'ELECTRONIQUE DE LA LIAISON ENTRE NOYAU ET M'ESOSOME CHEZ BACILLUS SUBTILIS. Ann Inst Pasteur (Paris) 1964 Sep;107:384–400. [PubMed] [Google Scholar]
  15. Rogers H. J. Bacterial growth and the cell envelope. Bacteriol Rev. 1970 Jun;34(2):194–214. doi: 10.1128/br.34.2.194-214.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shockman G. D. Symposium on the fine structure and replication of bacteria and their parts. IV. Unbalanced cell-wall synthesis: autolysis and cell-wall thickening. Bacteriol Rev. 1965 Sep;29(3):345–358. doi: 10.1128/br.29.3.345-358.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. VAN TUBERGEN R. P., SETLOW R. B. Quantitative radioautographic studies on exponentially growing cultures of Escherichia coli. The distribution of parental DNA, RNA, protein, and cell wall among progeny cells. Biophys J. 1961 Sep;1:589–625. doi: 10.1016/s0006-3495(61)86911-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WEIDEL W., PELZER H. BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS. Adv Enzymol Relat Areas Mol Biol. 1964;26:193–232. doi: 10.1002/9780470122716.ch5. [DOI] [PubMed] [Google Scholar]

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