Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Jan;109(1):379–384. doi: 10.1128/jb.109.1.379-384.1972

Gene Frequency Analysis of Chromosomal Initiation Sites in Bacillus subtilis after Ultraviolet Light or X-Ray Exposure

Daniel Billen 1, Gary Hellerman 1, Laura Carreira 1
PMCID: PMC247288  PMID: 4621630

Abstract

Bacillus subtilis was exposed to ultraviolet light (UV) or X rays, and gene frequency analysis was used to study the location of initiation sites of postirradiation deoxyribonucleic acid (DNA) synthesis. It was found that DNA synthesis resumes primarily from the origin after UV exposure. With X irradiation, the origin is not selectively replicated. Elevated origin-to-terminal marker ratios observed after UV exposure of exponentially growing cells were interpreted as evidence for selective UV resistance of the replicative origin region of the bacterial chromosome.

Full text

PDF
379

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anraku N., Landman O. E. Control of the synthesis of macromolecules during amino acid and thymine starvation in Bacillus subtilis. J Bacteriol. 1968 May;95(5):1813–1827. doi: 10.1128/jb.95.5.1813-1827.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Billen D., Carreira L. B. Characterization of DNA synthesis in an Hcr mutant of Escherichia coli exposed to ultraviolet light. Microbios. 1971 Mar;3(10):153–163. [PubMed] [Google Scholar]
  4. Billen D. Replication of the bacterial chromosome: location of new initiation sites after irradiation. J Bacteriol. 1969 Mar;97(3):1169–1175. doi: 10.1128/jb.97.3.1169-1175.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boyle J. V., Goss W. A., Cook T. M. Induction of excessive deoxyribonucleic acid synthesis in Escherichia coli by nalidixic acid. J Bacteriol. 1967 Nov;94(5):1664–1671. doi: 10.1128/jb.94.5.1664-1671.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doudney C O, Young C S. Ultraviolet Light Induced Mutation and Deoxyribonucleic Acid Replication in Bacteria. Genetics. 1962 Sep;47(9):1125–1138. doi: 10.1093/genetics/47.9.1125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  8. HANAWALT P. C., MAALOE O., CUMMINGS D. J., SCHAECHTER M. The normal DNA replication cycle. II. J Mol Biol. 1961 Apr;3:156–165. doi: 10.1016/s0022-2836(61)80042-9. [DOI] [PubMed] [Google Scholar]
  9. HAROLD F. M., ZIPORIN Z. Z. Synthesis of protein and of DNA in Escherichia coli irradiated with ultraviolet light. Biochim Biophys Acta. 1958 Aug;29(2):439–440. doi: 10.1016/0006-3002(58)90211-7. [DOI] [PubMed] [Google Scholar]
  10. Kallenbach N. R., Ma R. Initiation of deoxyribonucleic acid synthesis after thymine starvation of Bacillus subtilis. J Bacteriol. 1968 Feb;95(2):304–309. doi: 10.1128/jb.95.2.304-309.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LARK C., LARK K. G. EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI. J Mol Biol. 1964 Oct;10:120–136. doi: 10.1016/s0022-2836(64)80032-2. [DOI] [PubMed] [Google Scholar]
  12. Okubo S., Nakayama H. DNA synthesis after ultraviolet light irradiation in uv-sensitive mutants of Bacillus subtilis. Mutat Res. 1967 Sep-Oct;4(5):533–541. doi: 10.1016/0027-5107(67)90039-5. [DOI] [PubMed] [Google Scholar]
  13. PRITCHARD R. H., LARK K. G. INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI. J Mol Biol. 1964 Aug;9:288–307. doi: 10.1016/s0022-2836(64)80208-4. [DOI] [PubMed] [Google Scholar]
  14. Smith K. C., O'Leary M. E. Photoinduced DNA-protein cross-links and bacterial killing: a correlation at low temperatures. Science. 1967 Feb 24;155(3765):1024–1026. doi: 10.1126/science.155.3765.1024. [DOI] [PubMed] [Google Scholar]
  15. Stafford R. S., Donnellan J. E., Jr Photochemical evidence for conformation changes in DNA during germination of bacterial spores. Proc Natl Acad Sci U S A. 1968 Mar;59(3):822–828. doi: 10.1073/pnas.59.3.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stein G., Hanawalt P. Initiation of DNA replication cycles in Escherichia coli following DNA synthesis inhibition. J Mol Biol. 1969 Nov 28;46(1):135–144. doi: 10.1016/0022-2836(69)90061-8. [DOI] [PubMed] [Google Scholar]
  17. Tamir H., Gilvarg C. Density gradient centrifugation for the separation of sporulating forms of bacteria. J Biol Chem. 1966 Mar 10;241(5):1085–1090. [PubMed] [Google Scholar]
  18. Ward C. B., Hane M. W., Glaser D. A. Synchronous reinitiation of chromosome replication in E. coli B-r after nalidixic acid treatment. Proc Natl Acad Sci U S A. 1970 Jun;66(2):365–369. doi: 10.1073/pnas.66.2.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Worcel A. Induction of chromosome re-initiations in a thermosensitive DNA mutant of Escherichiacoli. J Mol Biol. 1970 Sep 14;52(2):371–386. doi: 10.1016/0022-2836(70)90037-9. [DOI] [PubMed] [Google Scholar]
  20. YOSHIKAWA H., SUEOKA N. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases. Proc Natl Acad Sci U S A. 1963 Apr;49:559–566. doi: 10.1073/pnas.49.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yoshikawa H., Haas M. On the regulation of the initiation of DNA replication in bacteria. Cold Spring Harb Symp Quant Biol. 1968;33:843–855. doi: 10.1101/sqb.1968.033.01.096. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES