Abstract
Gas chromatographic analysis of extracts from Bacillus caldolyticus and Bacillus caldotenax grown at 60, 70, or 80 C showed that both contain branched-chain fatty acids as major constituents at all temperatures tested. With increasing temperature, a decrease of i-C15 and an increase of i-C17 fatty acids were observed in both strains, as well as a decrease of i-C16 fatty acids corresponding to an increase of n-C16 fatty acids. The most obvious difference was that the shifts observed with B. caldolyticus occurred mainly upon raising the temperature to 10 C above, and in B. caldotenax upon lowering the temperature to 20 C below, the optimal growth temperature.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Daron H. H. Fatty acid composition of lipid extracts of a thermophilic Bacillus species. J Bacteriol. 1970 Jan;101(1):145–151. doi: 10.1128/jb.101.1.145-151.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunlap K. R., Perry J. J. Effect of substrate on the fatty acid composition of hydrocabon-utilizing microorganisms. J Bacteriol. 1967 Dec;94(6):1919–1923. doi: 10.1128/jb.94.6.1919-1923.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heinen W. Growth conditions and temperature-dependent substrate specificity of two extremely thermophilic bacteria. Arch Mikrobiol. 1971;76(1):2–17. doi: 10.1007/BF00409310. [DOI] [PubMed] [Google Scholar]
- Heinen W., Klein H. P., Volkmann C. M. Fatty acid composition of Thermus aquaticus at different growth temperatures. Arch Mikrobiol. 1970;72(2):199–202. doi: 10.1007/BF00409525. [DOI] [PubMed] [Google Scholar]
- JAMES A. T. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. Methods Biochem Anal. 1960;8:1–59. doi: 10.1002/9780470110249.ch1. [DOI] [PubMed] [Google Scholar]
- Shen P. Y., Coles E., Foote J. L., Stenesh J. Fatty acid distribution in mesophilic and thermophilic strains of the genus Bacillus. J Bacteriol. 1970 Aug;103(2):479–481. doi: 10.1128/jb.103.2.479-481.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]