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. 1972 Apr;110(1):126–132. doi: 10.1128/jb.110.1.126-132.1972

Ferredoxin- and Nicotinamide Adenine Dinucleotide-Dependent H2 Production from Ethanol and Formate in Extracts of S Organism Isolated from “Methanobacillus omelianskii”1

C Adinarayana Reddy a,2, M P Bryant a, M J Wolin a
PMCID: PMC247388  PMID: 4401599

Abstract

S organism ferments ethanol to acetate and H2 but grows poorly on ethanol unless the partial pressure of H2 is kept low, as when it is grown in combination with an H2-utilizing methanogenic bacterium. The present study shows that S organism contains an alcohol dehydrogenase and a formate dehydrogenase, both of which require nicotinamide adenine dinucleotide (NAD) for activity. Hydrogen is evolved from NADH generated by these activities via a ferredoxin-dependent oxidation of NADH to NAD and H2. NADH:NADP oxido-reductase activity was also demonstrated. The relationship of these activities to the growth of S organism is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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