Abstract
In a study of production of the common enterobacterial antigen (CA) by members of the Aeromonas group, ten strains of A. shigelloides, nine strains of A. hydrophila, and nine strains of A. salmonicida were used. Passive hemagglutination and hemolysis tests as well as the hemagglutination-inhibition procedure revealed that all strains of A. shigelloides, in contrast to the strains of the other two species, produce this antigenic determinant. The antigen of A. shigelloides was demonstrated even when the supernatant fluids of agar-grown cultures were used in a dilution of 1:1,000, whereas 10-times concentrated supernatant fluids obtained from the other two species were negative. Supernatant liquids of cultures of A. shigelloides failed to induce a significant CA immune response in rabbits; nonetheless, the animals were primed immunologically and responded with prompt production of CA antibodies in significant titers to a booster injection of a subeffective dose of CA obtained from Salmonella typhimurium. Strains of A. hydrophila and A. salmonicida neither induced CA antibody formation nor primed the animals. It is concluded that of the three species of the Aeromonas group only A. shigelloides, which may produce O antigen cross-reacting with Shigella sonnei and which has been isolated from patients with dysentery or gastroenteritis, produces CA. Production of this antigen, therefore, may help to characterize microorganisms belonging or related to the family Enterobacteriaceae.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BADER R. E. Uber die Herstellung eines agglutinierenden Serums gegen die Rundform von Shigella sonnei mit einem Stamm der Gattung Pseudomonas. Z Hyg Infektionskr. 1954;140(5):450–456. [PubMed] [Google Scholar]
- Diaz F., Neter E. Production of common enterobacterial antigen by pigment-producing, gram-negative bacilli. J Bacteriol. 1969 Jan;97(1):453–454. doi: 10.1128/jb.97.1.453-454.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferguson W. W., Henderson N. D. Description of Strain C27: A Motile Organism with the Major Antigen of Shigella sonnei Phase I. J Bacteriol. 1947 Aug;54(2):179–181. doi: 10.1128/jb.54.2.179-181.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUNIN C. M. SEPARATION, CHARACTERIZATION, AND BIOLOGICAL SIGNIFICANCE OF A COMMON ANTIGEN IN ENTEROBACTERIACEAE. J Exp Med. 1963 Oct 1;118:565–586. doi: 10.1084/jem.118.4.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayer H., Schmidt G. Hämagglutinine gegen ein gemeinsames Enterobacteriaceen-Antigen in E. coli R1-Antiseren. Zentralbl Bakteriol Orig. 1971;216(3):299–313. [PubMed] [Google Scholar]
- SAKAZAKI R., NAMIOKA S., NAKAYA R., FUKUMI H. Studies on so-called paracolon C27 (Ferguson). Jpn J Med Sci Biol. 1959 Oct;12:355–363. doi: 10.7883/yoken1952.12.355. [DOI] [PubMed] [Google Scholar]
- SUZUKI T., GORZYNSKI E. A., NETER E. SEPARATION BY ETHANOL OF COMMON AND SOMATIC ANTIGENS OF ENTEROBACTERIACEAE. J Bacteriol. 1964 Nov;88:1240–1243. doi: 10.1128/jb.88.5.1240-1243.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whang H. Y., Mayer H., Neter E. Differential effects on immunogenicity and antigenicity of heat, freezing and alkali treatment of bacterial antigens. J Immunol. 1971 Jun;106(6):1552–1558. [PubMed] [Google Scholar]
- Whang H. Y., Neter E. Further studies on effect of endotoxin on antibody response of rabbit to common antigen of Enterobacteriaceae. J Immunol. 1967 May;98(5):948–957. [PubMed] [Google Scholar]