Abstract
Kinetic parameters for three systems of active histidine uptake by germinated conidia of Neurospora crassa have been measured. Each system appears to follow typical Michaelis-Menten kinetics when studied separately from the other systems. Under the conditions studied, the general amino acid transport system was found to account for the major portion of histidine uptake from low concentrations. Three types of transport mutants with altered growth inhibition patterns were selected in a histidine auxotroph. Growth of one mutant, type basa, could be inhibited by the addition of methionine to a histidine-supplemented medium, and another type, neua, could be inhibited by the addition of arginine. These mutants were shown to be lacking active histidine uptake by the basic amino acid and neutral amino acid transport systems, respectively. Another type of double mutant (his-3, neur) could be inhibited only by the addition of very high concentrations of methionine in the presence of arginine and histidine, and the mutation appeared to have altered the specificity of the neutral amino acid permease.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAUERLE R. H., GARNER H. R. THE ASSIMILATION OF ARGININE AND LYSINE IN CANAVANINE RESISTANT AND SENSITIVE STRAINS OF NEUROSPORA CRASSA. Biochim Biophys Acta. 1964 Nov 8;93:316–322. doi: 10.1016/0304-4165(64)90381-2. [DOI] [PubMed] [Google Scholar]
- BROCKMAN H. E., DeBUSK A. G., WAGNER R. P. Mutant-associated antagonisms in Neurospora crassa. Arch Biochem Biophys. 1959 Oct;84:455–461. doi: 10.1016/0003-9861(59)90607-1. [DOI] [PubMed] [Google Scholar]
- Choke H. C. Mutants of NEUROSPORA CRASSA Permeable to Histidinol. Genetics. 1969 Aug;62(4):725–733. doi: 10.1093/genetics/62.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeBusk B. G., DeBusk A. G. Molecular transport in Neurospora crassa. I. Biochemical properties of a phenylalanine permease. Biochim Biophys Acta. 1965 Jun 15;104(1):139–150. doi: 10.1016/0304-4165(65)90229-1. [DOI] [PubMed] [Google Scholar]
- Haas F, Mitchell M B, Ames B N, Mitchell H K. A Series of Histidineless Mutants of Neurospora Crassa. Genetics. 1952 May;37(3):217–226. doi: 10.1093/genetics/37.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobson E. S., Metzenberg R. L. A new gene which affects uptake of neutral and acidic amino acids in Neurospora crassa. Biochim Biophys Acta. 1968 Feb 1;156(1):140–147. doi: 10.1016/0304-4165(68)90113-x. [DOI] [PubMed] [Google Scholar]
- MATHIESON M. J., CATCHESIDE D. G. Inhibition of histidine uptake in Neurospora crassa. J Gen Microbiol. 1955 Aug;13(1):72–83. doi: 10.1099/00221287-13-1-72. [DOI] [PubMed] [Google Scholar]
- Pall M. L. Amino acid transport in Neurospora crassa. 3. Acidic amino acid transport. Biochim Biophys Acta. 1970 Sep 15;211(3):513–520. doi: 10.1016/0005-2736(70)90256-7. [DOI] [PubMed] [Google Scholar]
- Pall M. L. Amino acid transport in Neurospora crassa. I. Properties of two amino acid transport systems. Biochim Biophys Acta. 1969 Jan 28;173(1):113–127. doi: 10.1016/0005-2736(69)90042-x. [DOI] [PubMed] [Google Scholar]
- Pall M. L., Kelly K. A. Specificity of transinhibition of amino acid transport in neurospora. Biochem Biophys Res Commun. 1971 Mar 5;42(5):940–947. doi: 10.1016/0006-291x(71)90521-3. [DOI] [PubMed] [Google Scholar]
- STLAWRENCE P., MALING B. D., ALTWERGER L., RACHMELER M. MUTATIONAL ALTERATION OF PERMEABILITY IN NEUROSPORA: EFFECTS ON GROWTH AND THE UPTAKE OF CERTAIN AMINO ACIDS AND RELATED COMPOUNDS. Genetics. 1964 Dec;50:1383–1402. doi: 10.1093/genetics/50.6.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schiltz J. R., Terry K. D. Nucleoside uptake during the germination of Neurospora crassa conidia. Biochim Biophys Acta. 1970;209(2):278–288. doi: 10.1016/0005-2787(70)90726-4. [DOI] [PubMed] [Google Scholar]
- Stadler D. R. Genetic control of the uptake of amino acids in Neurospora. Genetics. 1966 Aug;54(2):677–685. doi: 10.1093/genetics/54.2.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Teipel J., Koshland D. E., Jr The significance of intermediary plateau regions in enzyme saturation curves. Biochemistry. 1969 Nov;8(11):4656–4663. doi: 10.1021/bi00839a064. [DOI] [PubMed] [Google Scholar]
- Thwaites W. M., Pendyala L. Regulation of amino acid assimilation in a strain of Neurospora crassa lacking basic amino acid transport activity. Biochim Biophys Acta. 1969 Dec 30;192(3):455–461. doi: 10.1016/0304-4165(69)90394-8. [DOI] [PubMed] [Google Scholar]
- Tisdale J. H., DeBusk A. G. Developmental regulation of amino acid transport in Neurospora crassa. J Bacteriol. 1970 Nov;104(2):689–697. doi: 10.1128/jb.104.2.689-697.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wiley W. R., Matchett W. H. Tryptophan transport in Neurospora crassa. I. Specificity and kinetics. J Bacteriol. 1966 Dec;92(6):1698–1705. doi: 10.1128/jb.92.6.1698-1705.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodward V. W., De Zeeuw J. R., Srb A. M. THE SEPARATION AND ISOLATION OF PARTICULAR BIOCHEMICAL MUTANTS OF NEUROSPORA BY DIFFERENTIAL GERMINATION OF CONIDIA, FOLLOWED BY FILTRATION AND SELECTIVE PLATING. Proc Natl Acad Sci U S A. 1954 Mar;40(3):192–200. doi: 10.1073/pnas.40.3.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZALOKAR M. Kinetics of amino acid uptake and protein synthesis in Neurospora. Biochim Biophys Acta. 1961 Jan 29;46:423–432. doi: 10.1016/0006-3002(61)90573-x. [DOI] [PubMed] [Google Scholar]