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. 1972 Apr;110(1):430–438. doi: 10.1128/jb.110.1.430-438.1972

Electron Transport Systems of Nitrosomonas: Isolation of a Membrane-Envelope Fraction

A B Hooper 1, R H Erickson 1, K R Terry 1
PMCID: PMC247424  PMID: 4336111

Abstract

Freezing and thawing of Nitrosomonas, followed by centrifugation of the homogenate at 3,000 × g, resulted in a fraction which appeared to consist of an intact membrane-envelope complex and contained approximately 50% of the cell protein and more than 90% of the ubiquinone and cytochrome A-type mammalian cytochrome c oxidase activity. The supernatant fraction, resulting from subsequent centrifugation of the extract at 100,000 × g, contained approximately 50% of the cell protein and more than 80% of the B- and C-type cytochrome and P-463 and the enzymes glutamate dehydrogenase; hydroxylamine dehydrogenase; nitrite synthetase; nitrite reductase; and 2,6-dichlorophenolindophenol-, p-phenylenediamine-, pyrogallol-, and hydroquinone-oxidase. Data on the concentration of electron transport components in Nitrosomonas are presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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