Abstract
A stable L-form of Streptococcus faecalis (T53) was transferred in media containing decreasing concentrations of sucrose until it grew in medium without added osmotic stabilizer. This L-form (designated T53I) was compared with T53 from which it was derived. The survival of these two L-forms suspended at different osmolalities showed that maximal survival for T53I was 350 to 400 milliosmolal and for T53 was 900 to 1,000 milliosmolal. Both peaks were at the osmolality of their growth media. Measurement of intracellular potassium and sodium showed that the concentration of these ions was reduced in T53I.
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- Clasener H. A., Ensering H. L., Hijmans W. Persistance in mice of the L-phase of three streptococcal strains adapted to physiological osmotic conditions. J Gen Microbiol. 1970 Aug;62(2):195–202. doi: 10.1099/00221287-62-2-195. [DOI] [PubMed] [Google Scholar]
- DIENES L., SHARP J. T. The role of high electrolyte concentration in the production and growth of L forms of bacteria. J Bacteriol. 1956 Feb;71(2):208–213. doi: 10.1128/jb.71.2.208-213.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montgomerie J. Z., Kalmanson G. M., Guze L. B. Effect of osmotic stabilizer on protoplasts and bacterial forms of Streptococcus faecalis. J Lab Clin Med. 1967 Oct;70(4):539–553. [PubMed] [Google Scholar]
- Montgomerie J. Z., Potter C. S., Kalmanson G. M., Guze L. B. Penicillin: effect on sodium and potassium transport in bacterial and protoplast forms of Sterptococcus faecalis. Science. 1968 Mar 15;159(3820):1252–1254. doi: 10.1126/science.159.3820.1252. [DOI] [PubMed] [Google Scholar]
- Panos C., Cohen M., Fagan G. Lipid alterations after cell wall inhibition. Fatty acid content of Streptococcus pyogenes and derived L-form. Biochemistry. 1966 May;5(5):1461–1468. doi: 10.1021/bi00869a003. [DOI] [PubMed] [Google Scholar]
- SCHULTZ S. G., SOLOMON A. K. Cation transport in Escherichia coli. I. Intracellular Na and K concentrations and net cation movement. J Gen Physiol. 1961 Nov;45:355–369. doi: 10.1085/jgp.45.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]