Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 May;110(2):652–660. doi: 10.1128/jb.110.2.652-660.1972

Dissociation of Lac Messenger Ribonucleic Acid Transcription from Translation During Recovery from Inhibition of Protein Synthesis

Michael Artman a,1, Herbert L Ennis a
PMCID: PMC247461  PMID: 4553840

Abstract

Studies were made of the synthesis of Lac messenger ribonucleic acid (mRNA) by Escherichia coli in the absence of protein synthesis and of the coupling of transcription of lac operon to translation. Lac mRNA was not synthesized in the presence of chloramphenicol, and its synthesis steadily decreased during K+ deprivation and treatment with puromycin. Since under these conditions total mRNA synthesis is not inhibited it is suggested that the control of Lac mRNA is distinct from that which regulates total mRNA synthesis. Lac mRNA synthesized during recovery from K+ starvation or from chloramphenicol inhibition is not translated into functional enzyme, suggesting translational control over β-galactosidase synthesis.

Full text

PDF
652

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOREK E., RYAN A., ROCKENBACH J. Nucleic acid metabolism in relation to the lysogenic phenomenon. J Bacteriol. 1955 Apr;69(4):460–467. doi: 10.1128/jb.69.4.460-467.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen P. S., Ennis H. L. The requirement for potassium for bacteriophage T4 protein and deoxyribonucleic acid synthesis. Virology. 1965 Nov;27(3):282–289. doi: 10.1016/0042-6822(65)90107-8. [DOI] [PubMed] [Google Scholar]
  3. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ENNIS H. L., LUBIN M. Dissociation of ribonucleic acid and protein synthesis in bacteria deprived of potassium. Biochim Biophys Acta. 1961 Jun 24;50:399–402. doi: 10.1016/0006-3002(61)90355-9. [DOI] [PubMed] [Google Scholar]
  5. ENNIS H. L., LUBIN M. PRE-RIBOSOMAL PARTICLES FORMED IN POTASSIUM-DEPLETED CELLS. STUDIES ON DEGRADATION AND STABILIZATION. Biochim Biophys Acta. 1965 Apr 19;95:605–623. doi: 10.1016/0005-2787(65)90515-0. [DOI] [PubMed] [Google Scholar]
  6. Ennis H. L. Role of potassium in the regulation of polysome content and protein synthesis in Escherichia coli. Arch Biochem Biophys. 1971 Jan;142(1):190–200. doi: 10.1016/0003-9861(71)90275-x. [DOI] [PubMed] [Google Scholar]
  7. Ennis H. L., Sells B. H. Breakdown and re-formation of polysomes in Escherichia coli during inhibition of protein synthesis. Biochim Biophys Acta. 1968 Jul 23;161(2):503–508. doi: 10.1016/0005-2787(68)90126-3. [DOI] [PubMed] [Google Scholar]
  8. Fry M., Artman M. Studies of newly synthesized ribosomal ribonucleic acid of Escherichia coli. Biochem J. 1969 Nov;115(2):295–305. doi: 10.1042/bj1150295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GALE E. F., FOLKES J. P. The assimilation of amino-acids by bacteria. XV. Actions of antibiotics on nucleic acid and protein synthesis in Staphylococcus aureus. Biochem J. 1953 Feb;53(3):493–498. doi: 10.1042/bj0530493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Green M. H. Inactivation of the prophage lambda repressor without induction. J Mol Biol. 1966 Mar;16(1):134–148. doi: 10.1016/s0022-2836(66)80268-1. [DOI] [PubMed] [Google Scholar]
  11. Javor G. T., Ryan A., Borek E. Studies of the impaired inducibility in relaxed mutants of Escherichia coli. Biochim Biophys Acta. 1969 Oct 22;190(2):442–453. doi: 10.1016/0005-2787(69)90093-8. [DOI] [PubMed] [Google Scholar]
  12. KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
  13. Leive L., Kollin V. Controlling EDTA treatment to produce permeable Escherichia coli with normal metabolic processes. Biochem Biophys Res Commun. 1967 Jul 21;28(2):229–236. doi: 10.1016/0006-291x(67)90434-2. [DOI] [PubMed] [Google Scholar]
  14. MAGASANIK B. Catabolite repression. Cold Spring Harb Symp Quant Biol. 1961;26:249–256. doi: 10.1101/sqb.1961.026.01.031. [DOI] [PubMed] [Google Scholar]
  15. Monro R. E., Vazquez D. Ribosome-catalysed peptidyl transfer: effects of some inhibitors of protein synthesis. J Mol Biol. 1967 Aug 28;28(1):161–165. doi: 10.1016/s0022-2836(67)80085-8. [DOI] [PubMed] [Google Scholar]
  16. PARDEE A. B., PRESTIDGE L. S. The dependence of nucleic acid synthesis on the presence of amino acids in Escherichia coli. J Bacteriol. 1956 Jun;71(6):677–683. doi: 10.1128/jb.71.6.677-683.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perlman R. L., Pastan I. Regulation of beta-galactosidase synthesis in Escherichia coli by cyclic adenosine 3',5'-monophosphate. J Biol Chem. 1968 Oct 25;243(20):5420–5427. [PubMed] [Google Scholar]
  18. Ron E. Z., Kohler R. E., Davis B. D. Increased stability of polysomes in an Escherichia coli mutant with relaxed control of RNA synthesis. Proc Natl Acad Sci U S A. 1966 Aug;56(2):471–475. doi: 10.1073/pnas.56.2.471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Varmus H. E., Perlman R. L., Pastan I. Regulation of lac messenger ribonucleic acid synthesis by cyclic adenosine 3',5'-monophosphate and glucose. J Biol Chem. 1970 May 10;245(9):2259–2267. [PubMed] [Google Scholar]
  20. Varmus H. E., Perlman R. L., Pastan I. Regulation of lac transcription in antibiotic-treated E. coli. Nat New Biol. 1971 Mar 10;230(10):41–44. doi: 10.1038/newbio230041a0. [DOI] [PubMed] [Google Scholar]
  21. Weber M. J., DeMoss J. A. The inhibition by chloramphenicol of nascent protein formation in E. coli. Proc Natl Acad Sci U S A. 1966 May;55(5):1224–1230. doi: 10.1073/pnas.55.5.1224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES