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. 1972 May;110(2):699–705. doi: 10.1128/jb.110.2.699-705.1972

Isolation of Mycoplasma Membranes by Digitonin

Shlomo Rottem 1, Shmuel Razin 1
PMCID: PMC247467  PMID: 4112256

Abstract

The cell membrane of Mycoplasma hominis was isolated by lysing the cells with digitonin. Electron microscopy and chemical, density gradient, and electrophoretic analyses of the membrane proteins showed the membranes so obtained, like those isolated by osmotic lysis, to be relatively free of cytoplasmic contaminants. Sensitivity to digitonin lysis depended on temperature but was not affected by Mg2+ ions and was only slightly affected by the age of the culture. Accordingly, it seems that digitonin may be used for the isolation of cell membranes from sterol-requiring mycoplasmas that tend to be fairly resistant to osmotic lysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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