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. 1972 Jun;110(3):935–944. doi: 10.1128/jb.110.3.935-944.1972

Isolation and Characterization of a Phosphonomycin-Resistant Mutant of Escherichia coli K-12

P S Venkateswaran 1, H C Wu 1
PMCID: PMC247513  PMID: 4555418

Abstract

A mutant was isolated from Escherichia coli K-12 which showed increased resistance towards phosphonomycin, a new bactericidal antibiotic recently isolated from strains of Streptomyces. Evidence is presented which suggests that this mutant is resistant to lysis by phosphonomycin because of a lower affinity of phosphoenolpyruvate: uridine diphospho-N-acetylglucosamine enolpyruvyl transferase for this antibiotic. This mutant was also found to be temperature-sensitive in growth. At 42 C mutant cells grew poorly, and the rate of incorporation of 3H-diaminopimelic acid into trichloroacetic acid-insoluble material was also greatly reduced. Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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