Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Jun;110(3):1050–1057. doi: 10.1128/jb.110.3.1050-1057.1972

sai-1 Mutation in Saccharomyces cerevisiae: Characteristics of Inhibition by S-Adenosylmethionine and S-Adenosylhomocysteine and Protection by Methionine

Kemet D Spence 1, Stanley K Shapiro 1, Nancy K Hutson 1
PMCID: PMC247527  PMID: 4625121

Abstract

The relationship of methionine to the inhibition caused by S-adenosylmethionine and S-adenosylhomocysteine in strains containing the sai-1 mutation has been investigated and shown to affect indirectly the survival of the mutants. The ability of the mutants to take up both inhibitors is similar to that of the wild-type cells. The mutant also retains the ability to hydrolyze S-adenosylhomocysteine and incorporate the hydrolytic products into the various cellular fractions. Maximal inhibition of the sai-1 mutants occurs at an extracellular concentration of 0.005 mmS-adenosylmethionine and 0.025 to 0.05 mmS-adenosylhomocysteine when the cellular concentration is 0.05 mg (dry weight) per ml. The results suggest that the sai-1 mutation affects reaction(s) either not associated with methionine biosynthesis, or methionine synthesis and at least one other critical cellular function.

Full text

PDF
1050

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Botsford J. L., Jr, Parks L. W. Serine transhydroxymethylase in methionine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1969 Mar;97(3):1176–1183. doi: 10.1128/jb.97.3.1176-1183.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Botsford J. L., Parks L. W. Role of S-adenosylmethionine in methionine biosynthesis in yeast. J Bacteriol. 1967 Oct;94(4):966–971. doi: 10.1128/jb.94.4.966-971.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUERRE J. A., SCHLENK F. Formation and metabolism of S-adenosyl-L-homocysteine in yeast. Arch Biochem Biophys. 1962 Mar;96:575–579. doi: 10.1016/0003-9861(62)90339-9. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. OGUR M., ROSEN G. The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Arch Biochem. 1950 Feb;25(2):262–276. [PubMed] [Google Scholar]
  6. PIGG C. J., SORSOLI W. A., PARKS L. W. INDUCTION OF THE METHIONINE-ACTIVATING ENZYME IN SACCHAROMYCES CEREVISIAE. J Bacteriol. 1964 Apr;87:920–923. doi: 10.1128/jb.87.4.920-923.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SHAPIRO S. K., ALMENAS A., THOMSON J. F. BIOSYNTHESIS OF METHIONINE IN SACCHAROMYCES CEREVISIAE. KINETICS AND MECHANISM OF REACTION OF S-ADENOSYLMETHIONINE:HOMOCYSTEINE METHYLTRANSFERASE. J Biol Chem. 1965 Jun;240:2512–2518. [PubMed] [Google Scholar]
  8. SORSOLI W. A., SPENCE K. D., PARKS L. W. AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE. J Bacteriol. 1964 Jul;88:20–24. doi: 10.1128/jb.88.1.20-24.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SVIHLA G., DAINKO J. L., SCHLENK F. Ultraviolet microscopy of purine compounds in the yeast vacuole. J Bacteriol. 1963 Feb;85:399–409. doi: 10.1128/jb.85.2.399-409.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SVIHLA G., SCHLENK F. S-adenosylmethionine in the vacuole of Candida utilis. J Bacteriol. 1960 Jun;79:841–848. doi: 10.1128/jb.79.6.841-848.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schlenk F., Dainko J. L., Svihla G. The accumulation and intracellular distribution of biological sulfoninum compounds in yeast. Arch Biochem Biophys. 1970 Sep;140(1):228–236. doi: 10.1016/0003-9861(70)90027-5. [DOI] [PubMed] [Google Scholar]
  12. Schlenk F., Zydek C. R., Ehninger D. J., Dainko J. L. The production of S-adenosyl-L-methionine and S-adenosyl-L-ethionine by yeast. Enzymologia. 1965 Nov 6;29(3):283–298. [PubMed] [Google Scholar]
  13. Shapiro S. K., Ehninger D. J. Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine. Anal Biochem. 1966 May;15(2):323–333. doi: 10.1016/0003-2697(66)90038-8. [DOI] [PubMed] [Google Scholar]
  14. Spence K. D., Parks L. W., Shapiro S. K. Dominant mutation for ethionine resistance in Saccharomyces cerevisae. J Bacteriol. 1967 Nov;94(5):1531–1537. doi: 10.1128/jb.94.5.1531-1537.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spence K. D., Shapiro S. K. Some mutants of Saccharomyces cerevisiae inhibited by adenoylmethionine and adenosylhomocysteine. J Bacteriol. 1967 Oct;94(4):1136–1142. doi: 10.1128/jb.94.4.1136-1142.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]
  17. Zappia V., Zydek-Cwick R., Schlenk F. The specificity of S-adenosylmethionine derivatives in methyl transfer reactions. J Biol Chem. 1969 Aug 25;244(16):4499–4509. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES