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. 1972 Jun;110(3):1058–1064. doi: 10.1128/jb.110.3.1058-1064.1972

Isolation and Characterization of Ultraviolet Light-Sensitive Mutants of the Blue-Green Alga Anacystis nidulans

Yukio Asato a,1
PMCID: PMC247528  PMID: 4624108

Abstract

Three independently isolated ultraviolet light-sensitive (uvs) mutants of Anacystis nidulans were characterized. Strain uvs-1 was most sensitive to UV in the absence of photoreactivation. Pretreatment with caffeine suppressed the dark-survival curve of strain uvs-1, indicating the presence of excision enzymes involved in dark repair. Under “black” and “white” illumination, strain uvs-1 displays photoreactivation properties nearly comparable to wild-type culture. Mutants uvs-35 and uvs-88 appeared to have partial photorecovery capacities. Upon pretreatment with chloramphenicol, photoreactivation properties of strains uvs-1 and uvs-88 were not evident although the partial photoreactivation characteristics of strain uvs-35 remained the same. Data indicate that strains uvs-1, uvs-35, and uvs-88 are probably genetically distinct UV-sensitive mutants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asato Y., Folsome C. E. Mutagenesis of Anacystis nidulans by n-methyl-n'-nitro-N-nitroso-guanidine and UV irradiation. Mutat Res. 1969 Nov-Dec;8(3):531–536. doi: 10.1016/0027-5107(69)90070-0. [DOI] [PubMed] [Google Scholar]
  2. Asato Y., Folsome C. E. Temporal genetic mapping of the blue-green alga, Anacystis nidulans. Genetics. 1970 Jul;65(3):407–419. doi: 10.1093/genetics/65.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edelman M., Swinton D., Schiff J. A., Epstein H. T., Zeldin B. Deoxyribonucleic Acid of the blue-green algae (cyanophyta). Bacteriol Rev. 1967 Dec;31(4):315–331. doi: 10.1128/br.31.4.315-331.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanawalt P. C. The U.V. sensitivity of bacteria: its relation to the DNA replication cycle. Photochem Photobiol. 1966 Jan;5(1):1–12. [PubMed] [Google Scholar]
  5. JAGGER J., STAFFORD R. S. EVIDENCE FOR TWO MECHANISMS OF PHOTOREACTIVATION IN ESCHERICHIA COLI B. Biophys J. 1965 Jan;5:75–88. doi: 10.1016/s0006-3495(65)86703-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Roberts R. B., Aldous E. RECOVERY FROM ULTRAVIOLET IRRADIATION IN ESCHERICHIA COLI. J Bacteriol. 1949 Mar;57(3):363–375. doi: 10.1128/jb.57.3.363-375.1949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rupp W. D., Howard-Flanders P. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J Mol Biol. 1968 Jan 28;31(2):291–304. doi: 10.1016/0022-2836(68)90445-2. [DOI] [PubMed] [Google Scholar]
  8. Saito N., Werbin H. Purification of a blue-green algal deoxyribonucleic acid photoreactiving enzyme. An enzyme requiring light as a physical cofactor to perform its catalytic function. Biochemistry. 1970 Jun 23;9(13):2610–2620. doi: 10.1021/bi00815a008. [DOI] [PubMed] [Google Scholar]
  9. Setlow R. B., Carrier W. L. Formation and destruction of pyrimidine dimers in polynucleotides by ultra-violet irradiation in the presence of proflavine. Nature. 1967 Mar 4;213(5079):906–907. doi: 10.1038/213906a0. [DOI] [PubMed] [Google Scholar]
  10. Shimada K., Takagi Y. The effect of caffeine on the repair of ultraviolet-damaged DNA in bacteria. Biochim Biophys Acta. 1967;145(3):763–770. doi: 10.1016/0005-2787(67)90135-9. [DOI] [PubMed] [Google Scholar]
  11. Swain F. M. Paleomicrobiology. Annu Rev Microbiol. 1969;23:455–472. doi: 10.1146/annurev.mi.23.100169.002323. [DOI] [PubMed] [Google Scholar]
  12. Van Baalen C. The effects of ultraviolet irradiation on a coccoid blue-green alga: survival, photosynthesis, and photoreactivation. Plant Physiol. 1968 Oct;43(10):1689–1695. doi: 10.1104/pp.43.10.1689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Werbin H., Rupert C. S. Presence of photoreactivating enzyme in blue-green algal cells. Photochem Photobiol. 1968 Feb;7(2):225–230. doi: 10.1111/j.1751-1097.1968.tb08008.x. [DOI] [PubMed] [Google Scholar]
  14. Wu J. H., Lewin R. A., Werbin H. Photoreactivation of UV-irradiated blue-green algal virus LPP-1. Virology. 1967 Apr;31(4):657–664. doi: 10.1016/0042-6822(67)90194-8. [DOI] [PubMed] [Google Scholar]

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