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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 May;102(2):306–310. doi: 10.1128/jb.102.2.306-310.1970

Cholesterol Requirement of Mycoplasmas

Shmuel Razin a,1, Joseph G Tully b
PMCID: PMC247552  PMID: 4911537

Abstract

Cholesterol requirement for growth of mycoplasmas was tested in a serum-free medium supplemented with albumin, l-arginine, palmitic acid, and various concentrations of cholesterol dissolved in Tween 80. In cases in which Tween 80 was shown to inhibit growth, the test medium was supplemented with cholesterol dissolved in ethanol. Of the 31 species examined, all but Mycoplasma laidlawii, M. granularum, and Mycoplasma species strain S-743 exhibited a growth response to cholesterol. No requirement for cholesterol could be shown with the stable L-phase variants of Streptobacillus moniliformis and Proteus species. The results provide experimental support for the view that the large majority of the established Mycoplasma species require cholesterol for growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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