Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 May;102(2):411–422. doi: 10.1128/jb.102.2.411-422.1970

Physiological Basis of Transient Repression of Catabolic Enzymes in Escherichia coli

Bonnie Tyler a,1, Boris Magasanik a
PMCID: PMC247566  PMID: 4911541

Abstract

Transient repression of catabolic enzymes occurs in cells that encounter a new carbon compound in their growth medium, but only when the cells contain the enzyme catalyzing the transfer of phosphate from phosphoenolpyruvate to a small heat-stable protein (HPr), as well as a permease capable of transporting the new compound across the cell membrane. The newly added compound need not be metabolized. The degree and duration of the transient repression have no obvious relation to the intracellular level of the exogenously added compound. It is suggested that the actual passage of the compound through the cell membrane is responsible for the repression.

Full text

PDF
411

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASENSIO C., AVIGAD G., HORECKER B. L. PREFERENTIAL GALACTOSE UTILIZATION IN A MUTANT STRAIN OF E. COLI. Arch Biochem Biophys. 1963 Dec;103:299–309. doi: 10.1016/0003-9861(63)90419-3. [DOI] [PubMed] [Google Scholar]
  2. BOEZI J. A., COWIE D. B. Kinetic studies of beta-galactosidase induction. Biophys J. 1961 Nov;1:639–647. doi: 10.1016/s0006-3495(61)86913-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BUTTIN G., COHEN G. N., MONOD J., RICKENBERG H. V. La galactoside-perméase d'Escherichia coli. Ann Inst Pasteur (Paris) 1956 Dec;91(6):829–857. [PubMed] [Google Scholar]
  4. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. I. LA BIOSYNTH'ESE INDUITE DE LA GALACTOKINASE ET L'INDUCTION SIMULTAN'EE DE LA S'EQUENCE ENZYMATIQUE. J Mol Biol. 1963 Aug;7:164–182. doi: 10.1016/s0022-2836(63)80044-3. [DOI] [PubMed] [Google Scholar]
  5. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. II. LE D'ETERMINISME G'EN'ETIQUE DE LA R'EGULATION. J Mol Biol. 1963 Aug;7:183–205. doi: 10.1016/s0022-2836(63)80045-5. [DOI] [PubMed] [Google Scholar]
  6. Böck A., Neidhardt F. C. Isolation of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase Activity. J Bacteriol. 1966 Aug;92(2):464–469. doi: 10.1128/jb.92.2.464-469.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Böck A., Neidhardt F. C. Properties of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase. J Bacteriol. 1966 Aug;92(2):470–476. doi: 10.1128/jb.92.2.470-476.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cozzarelli N. R., Freedberg W. B., Lin E. C. Genetic control of L-alpha-glycerophosphate system in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):371–387. doi: 10.1016/0022-2836(68)90415-4. [DOI] [PubMed] [Google Scholar]
  9. Cozzarelli N. R., Koch J. P., Hayashi S., Lin E. C. Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli. J Bacteriol. 1965 Nov;90(5):1325–1329. doi: 10.1128/jb.90.5.1325-1329.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ECHOLS H., GAREN A., GAREN S., TORRIANI A. Genetic control of repression of alkaline phosphatase in E. coli. J Mol Biol. 1961 Aug;3:425–438. doi: 10.1016/s0022-2836(61)80055-7. [DOI] [PubMed] [Google Scholar]
  11. FRAENKEL D. G., FALCOZ-KELLY F., HORECKER B. L. THE UTILIZATION OF GLUCOSE 6-PHOSPHATE BY GLUCOKINASELESS AND WILD-TYPE STRAINS OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Nov;52:1207–1213. doi: 10.1073/pnas.52.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fraenkel D. G. Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. J Bacteriol. 1968 Apr;95(4):1267–1271. doi: 10.1128/jb.95.4.1267-1271.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ganesan A. K., Rotman B. Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease. J Mol Biol. 1966 Mar;16(1):42–50. doi: 10.1016/s0022-2836(66)80261-9. [DOI] [PubMed] [Google Scholar]
  14. HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. I. General properties as studied in a galactokinaseless mutant. J Biol Chem. 1960 Jun;235:1580–1585. [PubMed] [Google Scholar]
  15. HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. II. Characteristics of the exit process. J Biol Chem. 1960 Jun;235:1586–1590. [PubMed] [Google Scholar]
  16. KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LIN E. C., KOCH J. P., CHUSED T. M., JORGENSEN S. E. Utilization of L-alpha-glycerophosphate by Escherichia coli without hydrolysis. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2145–2150. doi: 10.1073/pnas.48.12.2145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LIN E. C., LERNER S. A., JORGENSEN S. E. A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes. Biochim Biophys Acta. 1962 Jul 2;60:422–424. doi: 10.1016/0006-3002(62)90423-7. [DOI] [PubMed] [Google Scholar]
  19. LOOMIS W. F., Jr, MAGASANIK B. THE RELATION OF CATABOLITE REPRESSION TO THE INDUCTION SYSTEM FOR BETA-GALACTOSIDASE IN ESCHERICHIA COLI. J Mol Biol. 1964 Mar;8:417–426. doi: 10.1016/s0022-2836(64)80205-9. [DOI] [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. Loomis W. F., Jr, Magasanik B. The catabolite repression gene of the lac operon in Escherichia coli. J Mol Biol. 1967 Feb 14;23(3):487–494. doi: 10.1016/s0022-2836(67)80120-7. [DOI] [PubMed] [Google Scholar]
  22. Moses V., Prevost C. Catabolite repression of beta-galactosidase synthesis in Escherichia coli. Biochem J. 1966 Aug;100(2):336–353. doi: 10.1042/bj1000336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. PRESTIDGE L. S., PARDEE A. B. A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI. Biochim Biophys Acta. 1965 May 4;100:591–593. doi: 10.1016/0304-4165(65)90029-2. [DOI] [PubMed] [Google Scholar]
  24. Paigen K. Phenomenon of transient repression in Escherichia coli. J Bacteriol. 1966 Mar;91(3):1201–1209. doi: 10.1128/jb.91.3.1201-1209.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Prevost C., Moses V. Pool sizes of metabolic intermediates and their relation to glucose repression of beta-galactosidase synthesis in Escherichia coli. Biochem J. 1967 May;103(2):349–357. doi: 10.1042/bj1030349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. ROTMAN B. Separate permeases for the accumulation of methyl-beta-D-galactoside and methyl-beta-D-thiogalactoside in Escherichia coli. Biochim Biophys Acta. 1959 Apr;32:599–601. doi: 10.1016/0006-3002(59)90659-6. [DOI] [PubMed] [Google Scholar]
  27. Rotman B., Ganesan A. K., Guzman R. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities. J Mol Biol. 1968 Sep 14;36(2):247–260. doi: 10.1016/0022-2836(68)90379-3. [DOI] [PubMed] [Google Scholar]
  28. SCHLESINGER M. J., LEVINTHAL C. Hybrid protein formation of E. coli alkaline phosphatase leading to in vitro complementation. J Mol Biol. 1963 Jul;7:1–12. doi: 10.1016/s0022-2836(63)80014-5. [DOI] [PubMed] [Google Scholar]
  29. Simoni R. D., Levinthal M., Kundig F. D., Kundig W., Anderson B., Hartman P. E., Roseman S. Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1963–1970. doi: 10.1073/pnas.58.5.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Tanaka S., Fraenkel D. G., Lin E. C. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem Biophys Res Commun. 1967 Apr 7;27(1):63–67. doi: 10.1016/s0006-291x(67)80040-8. [DOI] [PubMed] [Google Scholar]
  31. Tyler B., Loomis W. F., Jr, Magasanik B. Transient repression of the lac operon. J Bacteriol. 1967 Dec;94(6):2001–2011. doi: 10.1128/jb.94.6.2001-2011.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tyler B., Magasanik B. Molecular basis of transient repression of beta-galactosidase in Escherichia coli. J Bacteriol. 1969 Feb;97(2):550–556. doi: 10.1128/jb.97.2.550-556.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES