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. 2008 Jul;179(3):1357–1371. doi: 10.1534/genetics.108.090290

TABLE 1.

Cell migrations

ALMa
BDUb
CANc
HSNd
QLe
QRf:
Strain Anterior Posterior Anterior Posterior Anterior Posterior Anterior Posterior N Anterior Posterior N Posterior N
Wild typeg 3.1 0 0 0 1.5 0 0 1.5 65 0 0 57 3.1 32
Wnt mutants
cwn-1g 7.9 0 0 18.4 0 0 0 0 38 0 0 32 72.7 33
cwn-2g 2.6 0 0 44.7 23.7 0 0 0 38 0 0 30 46.7 30
egl-20g 3.3 0 0 0 0 0 0 80.0 30 96.3 0 54 93.8 32
lin-44g 0 0 0 0 0 0 0 0 31 0 0 29 7.7 37
mom-2g 0 0 0 0 0 0 0 0 28 0 0 36 0 36
lin-44; cwn-1 11.6 0 0 21.4 0 0 0 20.9 42 0 0 32 90.3 32
lin-44; egl-20 4.0 0 0 4.0 0 0 0 92.0 25 90.6 0 32 59.4 32
cwn-1; egl-20g 2.9 5.9 0 12.1 5.9 0 0 100 34 9.3 0 43 100 30
cwn-1; mom-2h 0 0 0 29.0 0 0 0 13.8 24 0 0 46 89.5 40
egl-20 cwn-2 3.3 5.0 0 55.0 28.3 11.7 0 84.6 59 100 0 48 95.5 44
cwn-1; cwn-2g 48.8 2.4 0 71.4 35.7 33.3 0 60.0 40 0 0 32 90.3 31
cwn-1; egl-20 cwn-2 34.9 4.7 2.3 39.5 59.1 2.3 0 95.2 42 5.0 65.0 40 100 28
lin-44; egl-20 cwn-2 4.0 0 22.0 34.0 32.0 10.0 0 93.5 50 100 0 30 100 30
egl-20 cwn-2; mom-2h 0 6.5 0 58.1 9.7 35.5 0 75.9 31 96.0 0 50 100 52
lin-44; cwn-1; egl-20 cwn-2 20.7 6.9 7.1 60.7 38.0 3.4 0 96.2 29 7.5 40.0 40 100 40
lin-44; cwn-1; egl-20 cwn-2; mom-2h ND ND ND ND ND ND ND ND 0 41.7 41.7 12 100 20
Candidate Wnt receptor mutants
cfz-2g 20.0 0 0 0 2.5 0 0 2.5 40 0 0 58 11.7 60
mig-1 2.5 0 0 0 0 0 0 63.6 23 50.7 5.5 73 3.1 52
lin-17 0 0 0 0 0 0 0 10.0 30 18.6 0 30 0 30
mom-5ghi 0 0 0 6.7 6.3 0 0 13.3 31 0 0 33 86.7 30
lin-18 12.5 0 0 0 3.1 0 0 0 64 0 0 42 2.3 44
cam-1g 27.1 0 0 14.1 85.7 0 65.2 0 64 0 0 43 38.5 39
mig-1 lin-17 0 0 0 0 0 0 0 37.1 36 30.3 12.1 34 0 36
lin-17; cfz-2 7.3 0 0 0 7.3 0 0 17.9 40 25.0 0 36 3.6 56
mom-5; cfz-2gh 3.2 0 0 3.4 29.0 0 0 0 31 0 0 43 100 30
mig-1; cfz-2 4.9 0 0 0 0 0 0 92.7 41 77.3 0 46 4.3 46
lin-17 mom-5 5.3 2.6 0 23.7 10.5 0 0 21.1 38 16.7 5.0 63 100 74
cfz-2; lin-18 4.5 0 0 2.3 13.6 0 0 0 54 0 0 38 0 40
cam-1; cfz-2 21.4 3.6 12.0 8.0 72.4 0 50.0 3.6 28 0 0 22 13.3 30
mig-1; cfz-2; lin-18 0 0 0 2.3 6.8 0 0 74.4 44 82.2 8.9 46 5.5 36
mig-1 lin-17; cfz-2 10.2 0 0 0 2.0 0 0 38.8 47 53.7 22.0 42 2.1 48
mig-1 lin-17 mom-5h 10.0 0 0 6.7 10.0 3.3 0 56.7 30 57.8 15.6 45 96.6 59
mig-1 lin-17 mom-5; cfz-2h 0 0 5.7 17.1 14.3 0 0 74.3 34 35.6 34.5 87 91.4 70
Wnt and candidate receptor mutants
egl-20; cfz-2g 5.3 0 0 0 5.3 0 0 72.2 36 90.6 0 32 56 25
lin-44; cfz-2 10.4 0 0 0 7.5 0 0 0 67 0 0 40 8.6 36
cwn-1; cfz-2g 2.2 0 0 15.2 2.2 0 0 4.3 46 0 0 42 94.3 35
cwn-2; cfz-2g 5.1 0 0 45.5 39.4 0 0 2.1 98 0 0 30 51.6 31
mom-5; cwn-1h 0 3.4 0 46.2 3.3 0 0 7.1 29 0 0 38 100 38
cam-1 cwn-1 28.6 0 2.5 30.0 90.9 0 41.5 2.4 40 0 0 36 69.0 30
cam-1; cwn-2 61.9 0 1.3 17.8 73.3 0 56.1 2.4 43 0 0 44 53.4 46
cam-1; egl-20 cwn-2 44.1 8.8 28.9 28.1 85.3 0 12.9 54.8 31 ND ND ND
lin-44; cwn-1; cfz-2 0 0 0 11.6 0 0 0 13.3 43 0 0 28 71.4 26
egl-20 cwn-2; cfz-2 7.0 0 7.1 59.5 60.5 2.3 0 79.5 41 100 0 38 100 30
cwn-1; cwn-2; cfz-2g 36.4 18.2 0 74 75.0 0 0 67.5 44 0 0 30 95.0 40
lin-44; egl-20 cwn-2; cfz-2 7.7 0 8.6 42.9 25.6 17.9 0 91.7 37 100 0 30 100 40
mab-5(gf)j 0 0 0 10.4 0 0 2.1 0 48 0 4.0 50 0 44
cwn-1; mab-5(gf); egl-20 cwn-2j 20.7 13.8 0 48.1 60.6 0 0 100 30 0 34.1 44 100 47

Cell positions were assessed by Nomarski optics. ALM, BDU, CAN, and HSN positions were determined in newly hatched hermaphrodite larvae (L1). QR- and QL-descendant positions were determined in older L1 stage hermaphrodites after the V cells had divided. Numbers are the percentage of cells that failed to migrate to their normal position. N, number of cells scored. ND, not determined.

a

An ALM was scored as anteriorly misplaced (Anterior) if its nucleus was anterior to the V2 nucleus and posteriorly misplaced (Posterior) if posterior to the V3 nucleus.

b

A BDU was scored as anteriorly misplaced (Anterior) if its nucleus was more than a V-cell diameter anterior to the V1 nucleus and posteriorly misplaced (Posterior) if posterior to the V1 nucleus.

c

A CAN was scored as anteriorly misplaced (Anterior) if its nucleus was anterior to the V3 nucleus and posteriorly misplaced (Posterior) if posterior to the V4 nucleus.

d

An HSN was scored as anteriorly misplaced (Anterior) if its nucleus was anterior to the V3 nucleus and posteriorly misplaced (Posterior) if posterior to the V4 nucleus.

e

A QL-cell descendant was scored as misplaced anteriorly (Anterior) if its nucleus was anterior to V4.p. and posteriorly misplaced (Posterior) if posterior to the V5.p nucleus. Because they occupy positions near each other, the data for SDQL and PVM were combined. The position of PQR, a third QL descendant, was not included because it migrates to a location near other nuclei with similar morphology.

f

A QR-cell descendant was scored as posteriorly misplaced (Posterior) if its nucleus was posterior to the V2.a nucleus. Because they occupy positions near each other, the data for SDQR and AVM were combined. The position of AQR, a third QR descendant, was not included because it migrates to a location near other nuclei with similar morphology.

g

Some of these data have been reported elsewhere (Kim and Forrester 2003; Zinovyeva and Forrester 2005). They are presented here for comparison.

h

These animals were homozygous mutant progeny of heterozygous parents. See materials and methods for more information.

i

These animals were homozygous mutant progeny of dpy-5 mom-5/nT1 parents. Cell migration is normal in dpy-5 homozygous mutant animals.

j

These animals were mutant for the gain-of-function mab-5(e1751) allele.