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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 May;102(2):548–551. doi: 10.1128/jb.102.2.548-551.1970

Isolation of Monosomics in Yeast

J Bruenn 1, R K Mortimer 1
PMCID: PMC247584  PMID: 5419266

Abstract

Tetraploid cultures of Saccharomyces cerevisiae triplex (A/A/A/a) at several loci were sporulated. All the diploid spores are expected to be homozygous (A/A) or heterozygous (A/a) and, hence, to have the dominant phenotype. Cells lacking one of the chromosomes (monosomics) may show the recessive phenotype (a/−). Therefore, spores that grew on complete medium but failed to grow on the relevant synthetic single-omission media were presumed to be monosomic. These isolates were further characterized by sporulation, and several stable monosomics were established.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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