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. 1970 Jun;102(3):636–641. doi: 10.1128/jb.102.3.636-641.1970

Effect of Putative Deoxyribonucleic Acid Inhibitors on Macromolecular Synthesis in Saccharomyces cerevisiae

C T Wehr a,1, R D Kudrna a,1, L W Parks a
PMCID: PMC247605  PMID: 4246878

Abstract

The effects of inhibitors of bacterial deoxyribonucleic acid (DNA) synthesis upon logarithmically growing cultures of Saccharomyces cerevisiae were investigated. Cell division, ribonucleic acid (RNA) synthesis, and DNA synthesis were measured after addition of nalidixic acid, fluorodeoxyuridine, or phenethyl alcohol to cultures of yeast growing in defined and complex media. Both nalidixic acid and fluorodeoxyuridine had only temporary effects on nucleic acid synthesis in cultures growing in defined medium, and little or no observable effect on cultures growing in complex medium. Neither compound inhibited colony formation on complex solid medium, although growth was slow on defined solid medium. Phenethyl alcohol caused complete inhibition of DNA synthesis, RNA synthesis, and cell division in cultures growing in defined medium. In cultures growing in complex medium, RNA synthesis and cell division were inhibited to a lesser extent. A slight increase in DNA was observed in the presence of the inhibitor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERRAH G., KONETZKA W. A. Selective and reversible inhibition of the synthesis of bacterial deoxyribonucleic acid by phenethyl alcohol. J Bacteriol. 1962 Apr;83:738–744. doi: 10.1128/jb.83.4.738-744.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyle J. V., Cook T. M., Goss W. A. Mechanism of action of nalidixic acid on Escherichia coli. Vi. Cell-free studies. J Bacteriol. 1969 Jan;97(1):230–236. doi: 10.1128/jb.97.1.230-236.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyle J. V., Goss W. A., Cook T. M. Induction of excessive deoxyribonucleic acid synthesis in Escherichia coli by nalidixic acid. J Bacteriol. 1967 Nov;94(5):1664–1671. doi: 10.1128/jb.94.5.1664-1671.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burns V. W. Effects of phenethyl alcohol on yeast cells. J Cell Physiol. 1968 Oct;72(2):97–107. doi: 10.1002/jcp.1040720204. [DOI] [PubMed] [Google Scholar]
  5. Cohen S. S., Flaks J. G., Barner H. D., Loeb M. R., Lichtenstein J. THE MODE OF ACTION OF 5-FLUOROURACIL AND ITS DERIVATIVES. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1004–1012. doi: 10.1073/pnas.44.10.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cook T. M., Brown K. G., Boyle J. V., Goss W. A. Bactericidal action of nalidixic acid on Bacillus subtilis. J Bacteriol. 1966 Nov;92(5):1510–1514. doi: 10.1128/jb.92.5.1510-1514.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cook T. M., Deitz W. H., Goss W. A. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J Bacteriol. 1966 Feb;91(2):774–779. doi: 10.1128/jb.91.2.774-779.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cook T. M., Goss W. A., Deitz W. H. Mechanism of Action of Nalidixic Acid on Escherichia coli V. Possible Mutagenic Effect. J Bacteriol. 1966 Feb;91(2):780–783. doi: 10.1128/jb.91.2.780-783.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ESPOSITO R. E., HOLLIDAY R. THE EFFECT OF 5-FLUORODEOXYURIDINE ON GENETIC REPLICATION AND MITOTIC CROSSING OVER IN SYNCHRONIZED CULTURES OF USTILAGO MAYDIS. Genetics. 1964 Nov;50:1009–1017. doi: 10.1093/genetics/50.5.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Esposito R. E. Genetic recombination in synchronized cultures of Saccharomyces cerevisiae. Genetics. 1968 Jun;59(2):191–210. doi: 10.1093/genetics/59.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GOSS W. A., DEITZ W. H., COOK T. M. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI. J Bacteriol. 1964 Oct;88:1112–1118. doi: 10.1128/jb.88.4.1112-1118.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. GOSS W. A., DEITZ W. H., COOK T. M. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS. J Bacteriol. 1965 Apr;89:1068–1074. doi: 10.1128/jb.89.4.1068-1074.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KISSANE J. M., ROBINS E. The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem. 1958 Jul;233(1):184–188. [PubMed] [Google Scholar]
  14. Kantor G. J., Deering R. A. Effect of nalidixic acid and hydroxyurea on division ability of Escherichia coli fil+ and lon- strains. J Bacteriol. 1968 Feb;95(2):520–530. doi: 10.1128/jb.95.2.520-530.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lark K. G., Lark C. Regulation of chromosome replication in Escherichia coli: a comparison of the effects of phenethyl alcohol treatment with those of amino acid starvation. J Mol Biol. 1966 Sep;20(1):9–19. doi: 10.1016/0022-2836(66)90113-6. [DOI] [PubMed] [Google Scholar]
  16. Lester G. Inhibition of Growth, Synthesis, and Permeability in Neurospora crassa by Phenethyl Alcohol. J Bacteriol. 1965 Jul;90(1):29–37. doi: 10.1128/jb.90.1.29-37.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Prevost C., Moses V. Action of phyenethyl alcohol on the synthesis of macromolecules in Escherichia coli. J Bacteriol. 1966 Apr;91(4):1446–1452. doi: 10.1128/jb.91.4.1446-1452.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ROSENKRANZ H. S., CARR H. S., ROSE H. M. PHENETHYL ALCOHOL. I. EFFECT ON MACROMOLECULAR SYNTHESIS OF ESCHERICHIA COLI. J Bacteriol. 1965 May;89:1354–1369. doi: 10.1128/jb.89.5.1354-1369.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Remsen C. C., Lundgren D. G., Slepecky R. A. Inhibition of the development of the spore septum and membranes in Bacillus cereus by beta-phenethyl alcohol. J Bacteriol. 1966 Jan;91(1):324–331. doi: 10.1128/jb.91.1.324-331.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Silver S., Wendt L. Mechanism of action of phenethyl alcohol: breakdown of the cellular permeability barrier. J Bacteriol. 1967 Feb;93(2):560–566. doi: 10.1128/jb.93.2.560-566.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. TREICK R. W., KONETZKA W. A. PHYSIOLOGICAL STATE OF ESCHERICHIA COLI AND THE INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS BY PHENETHYL ALCOHOL. J Bacteriol. 1964 Dec;88:1580–1584. doi: 10.1128/jb.88.6.1580-1584.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wehr C. T., Parks L. W. Macromolecular synthesis in Saccharomyces cerevisiae in different growth media. J Bacteriol. 1969 May;98(2):458–466. doi: 10.1128/jb.98.2.458-466.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]
  24. Yura T., Wada C. Phenethyl alcohol resistance in Escherichia coli. I. Resistance of strain C600 and its relation to azide resistance. Genetics. 1968 Jun;59(2):177–190. doi: 10.1093/genetics/59.2.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Zahn R. K., Heicke B., Ochs H. G., Tiesler E., Forster W., Hanske W., Walter W., Hollstein H. In vitro inhibition of the synthesis of deoxyribonucleic acid by 2-phenyl-ethanol and some of its derivatives. Nature. 1966 Oct 15;212(5059):297–298. doi: 10.1038/212297a0. [DOI] [PubMed] [Google Scholar]

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