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. 1970 Jun;102(3):661–665. doi: 10.1128/jb.102.3.661-665.1970

Gene Expression After Transformation in Bacillus subtilis

Lawrence A Chasin a,1, Boris Magasanik a
PMCID: PMC247609  PMID: 4988038

Abstract

Sensitive assays of histidase activity were used to follow the production of this enzyme as directed by a gene newly introduced into cells of Bacillus subtilis by transformation. Histidase activity can be detected in histidase-negative recipient cells within 1 hr after the addition of deoxyribonucleic acid extracted from histidase-positive donors. Enzyme production continues for one to two additional hours and then ceases. Histidase production in the transformed cells is fully sensitive to catabolite repression. Catabolite repression is rapidly established after transformation of recipient cells that are resistant to this form of regulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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