Abstract
Teichoic acid-like material extracted by cold trichloroacetic acid from lyophilized whole cells of streptococci from groups A,D,E,O, and T was shown to give a positive precipitin reaction with group antisera. Similar material from cells of groups B,C,F,G,H,K,L,M,N,P,Q,R, and S did not give a positive reaction with group antisera. The group A material also reacted with anti-E serum; however, the opposite did not occur. A similar result was also obtained on the group T material and anti-O serum. The group A teichoic acid was purified by Sephadex column chromatography, and was shown to be free of cell wall peptidoglycan and polysaccharide, and ribitol teichoic acid. It was composed of glycerol, phosphate, alanine, and glucosamine. Alkaline hydrolysis showed the presence of ester-linked alanine and glucosaminylglycerol. Phosphorus was released from ester linkage by alkaline phosphatase. N-acetylglucosamine produced a 72% inhibition of the precipitin test at a level of 10 μmoles, and d-alanine methyl ester was significantly stronger than the l-alanine ester. A single precipitin band was seen with group A serum. The data indicate that teichoic acid of group A streptococci is a polymer composed of glycerol phosphate and containing N-acetylglucosamine and alanine. Antisera to these streptococci contain antibodies specific for the alanine and the glucosamine linkages. The use of serum containing antibodies to alanine-polyglycerophosphate shows that the occurrence of this type of teichoic acid is widespread among the streptococci.
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Selected References
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- Archibald A. R., Baddiley J., Button D., Heptinstall S., Stafford G. H. Ocuurrence of polymers containing N-acetylglucosamine-1-phosphate in bacterial walls. Nature. 1968 Aug 24;219(5156):855–856. doi: 10.1038/219855a0. [DOI] [PubMed] [Google Scholar]
- Archibald A. R., Baddiley J., Button D. The glycerol teichoic acid of walls of Staphylococcus lactis I3. Biochem J. 1968 Dec;110(3):543–557. doi: 10.1042/bj1100543. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Archibald A. R., Baddiley J. The teichoic acids. Adv Carbohydr Chem Biochem. 1966;21:323–375. doi: 10.1016/s0096-5332(08)60320-3. [DOI] [PubMed] [Google Scholar]
- Burger M. M. Teichoic acids: antigenic determinants, chain separation, and their location in the cell wall. Proc Natl Acad Sci U S A. 1966 Sep;56(3):910–917. doi: 10.1073/pnas.56.3.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CONSDEN R., GORDON A. H. Effect of salt on partition chromatograms. Nature. 1948 Jul 31;162(4109):180–180. doi: 10.1038/162180a0. [DOI] [PubMed] [Google Scholar]
- CRITCHLEY P., ARCHIBALD A. R., BADDILEY J. The intracellular teichoic acid from Lactobacillus arabinosus 17-5. Biochem J. 1962 Dec;85:420–431. doi: 10.1042/bj0850420. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ELLIOTT S. D. TEICHOIC ACID AND THE GROUP ANTIGEN OF LACTIC STREPTOCOCCI (GROUP N). Nature. 1963 Dec 21;200:1184–1185. doi: 10.1038/2001184a0. [DOI] [PubMed] [Google Scholar]
- HESS E. L., SLADE H. D. An electrophoretic examination of cell-free extracts from various serological types of group A hemolytic streptococci. Biochim Biophys Acta. 1955 Mar;16(3):346–353. doi: 10.1016/0006-3002(55)90237-7. [DOI] [PubMed] [Google Scholar]
- MCCARTY M. THE ROLE OF D-ALANINE IN THE SEROLOGICAL SPECIFICITY OF GROUP A STREPTOCOCCAL GLYCEROL TEICHOIC ACID. Proc Natl Acad Sci U S A. 1964 Aug;52:259–265. doi: 10.1073/pnas.52.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCARTY M. The occurrence of polyglycerophosphate as an antigenic component of various gram-positive bacterial species. J Exp Med. 1959 Apr 1;109(4):361–378. doi: 10.1084/jem.109.4.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
- RAJBHANDARY U. L., BADDILEY J. The intracellular teichoic acid from Staphylococcus aureus H. Biochem J. 1963 May;87:429–435. doi: 10.1042/bj0870429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHOCKMAN G. D., SLADE H. D. THE CELLULAR LOCATION OF THE STREPTOCOCCAL GROUP D ANTIGEN. J Gen Microbiol. 1964 Dec;37:297–305. doi: 10.1099/00221287-37-3-297. [DOI] [PubMed] [Google Scholar]
- SLADE H. D., SLAMP W. C. Cell-wall composition and the grouping antigens of Streptococci. J Bacteriol. 1962 Aug;84:345–351. doi: 10.1128/jb.84.2.345-351.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slade H. D. Extraction of Cell-Wall Polysaccharide Antigen from Streptococci. J Bacteriol. 1965 Sep;90(3):667–672. doi: 10.1128/jb.90.3.667-672.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swift H. F., Wilson A. T., Lancefield R. C. TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES. J Exp Med. 1943 Aug 1;78(2):127–133. doi: 10.1084/jem.78.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
- WICKEN A. J., BADDILEY J. Structure of intracellular teichoic acids from group D streptococci. Biochem J. 1963 Apr;87:54–62. doi: 10.1042/bj0870054. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WICKEN A. J., ELLIOTT S. D., BADDILEY J. The identity of streptococcal group D antigen with teichoic acid. J Gen Microbiol. 1963 May;31:231–239. doi: 10.1099/00221287-31-2-231. [DOI] [PubMed] [Google Scholar]
- WILSON A. T., WILEY G. G. THE CELLULAR ANTIGENS OF GROUP A STREPTOCOCCI; IMMUNOELECTROPHORETIC STUDIES OF THE C, M, T, PGP, E4, F, AND E ANTIGENS OF SEROTYPE 17 STREPTOCOCCI. J Exp Med. 1963 Oct 1;118:527–556. doi: 10.1084/jem.118.4.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willers J. M., Deddish P. A., Slade H. D. Transformation of type polysaccharide antigen synthesis and hemolysin synthesis in streptococci. J Bacteriol. 1968 Oct;96(4):1225–1230. doi: 10.1128/jb.96.4.1225-1230.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]