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. 1989 Feb;63(2):753–758. doi: 10.1128/jvi.63.2.753-758.1989

Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.

L Raptis 1, J B Bolen 1
PMCID: PMC247747  PMID: 2463382

Abstract

Polyomavirus middle tumor antigen (mT) was expressed in a line of mouse NIH 3T3 cells under control of the dexamethasone-regulatable mouse mammary tumor virus promotor. Contrary to rat F111 cells which were rendered anchorage independent by mT expression alone (L. Raptis, H. Lamfrom, and T.L. Benjamin, Mol. Cell. Biol. 5:2476-2487, 1985), mT-producing NIH 3T3 cells were unable to grow in agar even after full mT induction. The mT:pp60c-src-associated phosphatidylinositol kinase was activated in these cells to a degree similar to that in fully transformed cells expressing the small and large T antigens, in addition to mT. We therefore propose that the stimulation of this phosphatidylinositol kinase, although apparently necessary, is not sufficient for transformation of NIH 3T3 cells by polyomavirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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