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. 1989 Feb;63(2):952–954. doi: 10.1128/jvi.63.2.952-954.1989

Mutations located on both F1 and F2 subunits of the Newcastle disease virus fusion protein confer resistance to neutralization with monoclonal antibodies.

C Neyt 1, J Geliebter 1, M Slaoui 1, D Morales 1, G Meulemans 1, A Burny 1
PMCID: PMC247772  PMID: 2463386

Abstract

The fusion gene sequence of six Newcastle disease virus escape mutants revealed that residues important for the integrity of antigenic site 1 and antigenic site 2 were located, respectively, on the F2 subunit and within the cysteine-rich domain of the F1 subunit. We further report the antibody-binding capacity of these mutants.

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Selected References

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  1. Barrett T., Clarke D. K., Evans S. A., Rima B. K. The nucleotide sequence of the gene encoding the F protein of canine distemper virus: a comparison of the deduced amino acid sequence with other paramyxoviruses. Virus Res. 1987 Nov;8(4):373–386. doi: 10.1016/0168-1702(87)90009-8. [DOI] [PubMed] [Google Scholar]
  2. Blondel B., Crainic R., Fichot O., Dufraisse G., Candrea A., Diamond D., Girard M., Horaud F. Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibody-binding site. J Virol. 1986 Jan;57(1):81–90. doi: 10.1128/jvi.57.1.81-90.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brasseur R., Lorge P., Goormaghtigh E., Ruysschaert J. M., Espion D., Burny A. The mode of insertion of the paramyxovirus F1 N-terminus into lipid matrix, an initial step in host cell/virus fusion. Virus Genes. 1988 Jul;1(4):325–332. doi: 10.1007/BF00257096. [DOI] [PubMed] [Google Scholar]
  4. Chambers P., Millar N. S., Emmerson P. T. Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle disease virus. J Gen Virol. 1986 Dec;67(Pt 12):2685–2694. doi: 10.1099/0022-1317-67-12-2685. [DOI] [PubMed] [Google Scholar]
  5. Espion D., de Henau S., Letellier C., Wemers C. D., Brasseur R., Young J. F., Gross M., Rosenberg M., Meulemans G., Burny A. Expression at the cell surface of native fusion protein of the Newcastle disease virus (NDV) strain Italien from cloned cDNA. Arch Virol. 1987;95(1-2):79–95. doi: 10.1007/BF01311336. [DOI] [PubMed] [Google Scholar]
  6. Kawaoka Y., Naeve C. W., Webster R. G. Is virulence of H5N2 influenza viruses in chickens associated with loss of carbohydrate from the hemagglutinin? Virology. 1984 Dec;139(2):303–316. doi: 10.1016/0042-6822(84)90376-3. [DOI] [PubMed] [Google Scholar]
  7. Le L., Brasseur R., Wemers C., Meulemans G., Burny A. Fusion (F) protein gene of Newcastle disease virus: sequence and hydrophobicity comparative analysis between virulent and avirulent strains. Virus Genes. 1988 Jul;1(4):333–350. doi: 10.1007/BF00257097. [DOI] [PubMed] [Google Scholar]
  8. Long L., Portetelle D., Ghysdael J., Gonze M., Burny A., Meulemans G. Monoclonal antibodies to hemagglutinin-neuraminidase and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity. J Virol. 1986 Mar;57(3):1198–1202. doi: 10.1128/jvi.57.3.1198-1202.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGinnes L. W., Morrison T. G. Nucleotide sequence of the gene encoding the Newcastle disease virus fusion protein and comparisons of paramyxovirus fusion protein sequences. Virus Res. 1986 Sep;5(4):343–356. doi: 10.1016/0168-1702(86)90028-6. [DOI] [PubMed] [Google Scholar]
  10. Meulemans G., Gonze M., Carlier M. C., Petit P., Burny A., Long L. E. Pathogenicity of antigenic variants of Newcastle disease virus Italian strain selected with monoclonal antibodies. Ann Rech Vet. 1987;18(4):371–374. [PubMed] [Google Scholar]
  11. Millar N. S., Chambers P., Emmerson P. T. Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains. J Gen Virol. 1988 Mar;69(Pt 3):613–620. doi: 10.1099/0022-1317-69-3-613. [DOI] [PubMed] [Google Scholar]
  12. Morrison T., Ward L. J., Semerjian A. Intracellular processing of the Newcastle disease virus fusion glycoprotein. J Virol. 1985 Mar;53(3):851–857. doi: 10.1128/jvi.53.3.851-857.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nagai Y., Klenk H. D., Rott R. Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology. 1976 Jul 15;72(2):494–508. doi: 10.1016/0042-6822(76)90178-1. [DOI] [PubMed] [Google Scholar]
  14. Nicolaisen-Strouss K., Kumar H. P., Fitting T., Grant C. K., Elder J. H. Natural feline leukemia virus variant escapes neutralization by a monoclonal antibody via an amino acid change outside the antibody-binding epitope. J Virol. 1987 Nov;61(11):3410–3415. doi: 10.1128/jvi.61.11.3410-3415.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pellett P. E., Kousoulas K. G., Pereira L., Roizman B. Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants. J Virol. 1985 Jan;53(1):243–253. doi: 10.1128/jvi.53.1.243-253.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reeve P., Poste G. Studies on the cytopathogenicity of Newcastle disease virus: relation between virulence, polykaryocytosis and plaque size. J Gen Virol. 1971 Apr;11(1):17–24. doi: 10.1099/0022-1317-11-1-17. [DOI] [PubMed] [Google Scholar]
  17. Richardson C. D., Choppin P. W. Oligopeptides that specifically inhibit membrane fusion by paramyxoviruses: studies on the site of action. Virology. 1983 Dec;131(2):518–532. doi: 10.1016/0042-6822(83)90517-2. [DOI] [PubMed] [Google Scholar]
  18. Richardson C. D., Scheid A., Choppin P. W. Specific inhibition of paramyxovirus and myxovirus replication by oligopeptides with amino acid sequences similar to those at the N-termini of the F1 or HA2 viral polypeptides. Virology. 1980 Aug;105(1):205–222. doi: 10.1016/0042-6822(80)90168-3. [DOI] [PubMed] [Google Scholar]
  19. Sato H., Oh-hira M., Ishida N., Imamura Y., Hattori S., Kawakita M. Molecular cloning and nucleotide sequence of P, M and F genes of Newcastle disease virus avirulent strain D26. Virus Res. 1987 May;7(3):241–255. doi: 10.1016/0168-1702(87)90031-1. [DOI] [PubMed] [Google Scholar]
  20. Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]
  21. Scheid A., Choppin P. W. Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology. 1977 Jul 1;80(1):54–66. doi: 10.1016/0042-6822(77)90380-4. [DOI] [PubMed] [Google Scholar]
  22. Stave J. W., Card J. L., Morgan D. O., Vakharia V. N. Neutralization sites of type O1 foot-and-mouth disease virus defined by monoclonal antibodies and neutralization-escape virus variants. Virology. 1988 Jan;162(1):21–29. doi: 10.1016/0042-6822(88)90390-x. [DOI] [PubMed] [Google Scholar]
  23. Toyoda T., Sakaguchi T., Imai K., Inocencio N. M., Gotoh B., Hamaguchi M., Nagai Y. Structural comparison of the cleavage-activation site of the fusion glycoprotein between virulent and avirulent strains of Newcastle disease virus. Virology. 1987 May;158(1):242–247. doi: 10.1016/0042-6822(87)90261-3. [DOI] [PubMed] [Google Scholar]
  24. Waterson A. P., Pennington T. H., Allan W. H. Virulence in Newcastle disease virus. A preliminary study. Br Med Bull. 1967 May;23(2):138–143. doi: 10.1093/oxfordjournals.bmb.a070534. [DOI] [PubMed] [Google Scholar]
  25. Webster R. G., Rott R. Influenza virus A pathogenicity: the pivotal role of hemagglutinin. Cell. 1987 Aug 28;50(5):665–666. doi: 10.1016/0092-8674(87)90321-7. [DOI] [PubMed] [Google Scholar]
  26. Wiley D. C., Wilson I. A., Skehel J. J. Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature. 1981 Jan 29;289(5796):373–378. doi: 10.1038/289373a0. [DOI] [PubMed] [Google Scholar]
  27. Yewdell J. W., Gerhard W. Antigenic characterization of viruses by monoclonal antibodies. Annu Rev Microbiol. 1981;35:185–206. doi: 10.1146/annurev.mi.35.100181.001153. [DOI] [PubMed] [Google Scholar]

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