Abstract
The polycistronic P/C mRNA of Sendai virus is translated under cell-free conditions into five proteins (P, C', C, Y1, and Y2) from overlapping reading frames. In this study, we showed that in addition to the P, C', and C proteins, Y1 and Y2 were expressed by six different Sendai virus strains in infected cells. The Y proteins exhibited strain-specific variation in their gel mobility which corresponds to the variation seen in the cognate C proteins. While the relative levels of the P, C', and C proteins were consistent among various cell lines, the levels of Y1 and Y2 proteins varied among the cell lines used for viral infection.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baker S. C., Moyer S. A. Encapsidation of Sendai virus genome RNAs by purified NP protein during in vitro replication. J Virol. 1988 Mar;62(3):834–838. doi: 10.1128/jvi.62.3.834-838.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Banerjee A. K. Transcription and replication of rhabdoviruses. Microbiol Rev. 1987 Mar;51(1):66–87. doi: 10.1128/mr.51.1.66-87.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chinchar V. G., Portner A. Functions of Sendai virus nucleocapsid polypeptides: enzymatic activities in nucleocapsids following cleavage of polypeptide P by Staphylococcus aureus protease V8. Virology. 1981 Feb;109(1):59–71. doi: 10.1016/0042-6822(81)90471-2. [DOI] [PubMed] [Google Scholar]
- Davis N. L., Arnheiter H., Wertz G. W. Vesicular stomatitis virus N and NS proteins form multiple complexes. J Virol. 1986 Sep;59(3):751–754. doi: 10.1128/jvi.59.3.751-754.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deshpande K. L., Portner A. Monoclonal antibodies to the P protein of Sendai virus define its structure and role in transcription. Virology. 1985 Jan 15;140(1):125–134. doi: 10.1016/0042-6822(85)90451-9. [DOI] [PubMed] [Google Scholar]
- Dillon P. J., Gupta K. C. Early steps in the assembly of vesicular stomatitis virus nucleocapsids in infected cells. J Virol. 1988 May;62(5):1582–1589. doi: 10.1128/jvi.62.5.1582-1589.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Etkind P. R., Cross R. K., Lamb R. A., Merz D. C., Choppin P. W. In vitro synthesis of structural and nonstructural proteins of Sendai and SV5 viruses. Virology. 1980 Jan 15;100(1):22–33. doi: 10.1016/0042-6822(80)90548-6. [DOI] [PubMed] [Google Scholar]
- Giorgi C., Blumberg B. M., Kolakofsky D. Sendai virus contains overlapping genes expressed from a single mRNA. Cell. 1983 Dec;35(3 Pt 2):829–836. doi: 10.1016/0092-8674(83)90115-0. [DOI] [PubMed] [Google Scholar]
- Gupta K. C. Antisense oligodeoxynucleotides provide insight into mechanism of translation initiation of two Sendai virus mRNAs. J Biol Chem. 1987 Jun 5;262(16):7492–7496. [PubMed] [Google Scholar]
- Gupta K. C., Patwardhan S. ACG, the initiator codon for a Sendai virus protein. J Biol Chem. 1988 Jun 25;263(18):8553–8556. [PubMed] [Google Scholar]
- Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125–8148. doi: 10.1093/nar/15.20.8125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Patwardhan S., Gupta K. C. Translation initiation potential of the 5' proximal AUGs of the polycistronic P/C mRNA of Sendai virus. A multipurpose vector for site-specific mutagenesis. J Biol Chem. 1988 Apr 5;263(10):4907–4913. [PubMed] [Google Scholar]
- Portner A., Gupta K. C., Seyer J. M., Beachey E. H., Kingsbury D. W. Localization and characterization of Sendai virus nonstructural C and C' proteins by antibodies against synthetic peptides. Virus Res. 1986 Nov;6(2):109–121. doi: 10.1016/0168-1702(86)90043-2. [DOI] [PubMed] [Google Scholar]
- Shioda T., Hidaka Y., Kanda T., Shibuta H., Nomoto A., Iwasaki K. Sequence of 3,687 nucleotides from the 3' end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins. Nucleic Acids Res. 1983 Nov 11;11(21):7317–7330. doi: 10.1093/nar/11.21.7317. [DOI] [PMC free article] [PubMed] [Google Scholar]