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. 1989 Feb;63(2):992–996. doi: 10.1128/jvi.63.2.992-996.1989

Effect of Sindbis virus infection on induction of heat shock proteins in Aedes albopictus cells.

J Tatem 1, V Stollar 1
PMCID: PMC247782  PMID: 2911128

Abstract

When Aedes albopictus cells (clone C7) were infected with Sindbis virus, the production of cytopathic effect CPE depended largely on the conditions under which the cells were cultured. We observed marked inhibition of cellular RNA and protein synthesis, as well as a loss of the ability to induce heat shock proteins, e.g., hsp70, under conditions which led to cytopathic effect. Infected cells in which heat shock proteins could no longer be induced contained much lower amounts of hsp70 mRNA after heat shock than did mock-infected cells which were similarly treated. It is suggested that this decreased level of hsp70 mRNA is due to a failure of these cells to synthesize hsp70 mRNA after heat shock.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davey M. W., Dalgarno L. Semliki Forest virus replication in cultured Aedes albopictus cells: studies on the establishment of persistence. J Gen Virol. 1974 Sep;24(3):453–463. doi: 10.1099/0022-1317-24-3-453. [DOI] [PubMed] [Google Scholar]
  2. Durbin R. K., Stollar V. Sindbis virus mutants able to replicate in methionine-deprived Aedes albopictus cells. Virology. 1985 Jul 30;144(2):529–533. doi: 10.1016/0042-6822(85)90294-6. [DOI] [PubMed] [Google Scholar]
  3. Francoeur A. M., Poliquin L., Stanners C. P. The isolation of interferon-inducing mutants of vesicular stomatitis virus with altered viral P function for the inhibition of total protein synthesis. Virology. 1987 Sep;160(1):236–245. doi: 10.1016/0042-6822(87)90065-1. [DOI] [PubMed] [Google Scholar]
  4. Garry R. F., Ulug E. T., Bose H. R., Jr Induction of stress proteins in Sindbis virus- and vesicular stomatitis virus-infected cells. Virology. 1983 Sep;129(2):319–332. doi: 10.1016/0042-6822(83)90171-x. [DOI] [PubMed] [Google Scholar]
  5. Gillies S., Stollar V. Conditions necessary for inhibition of protein synthesis and production of cytopathic effect in Aedes albopictus cells infected with vesicular stomatitis virus. Mol Cell Biol. 1982 Jan;2(1):66–75. doi: 10.1128/mcb.2.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Lindquist S. The heat-shock response. Annu Rev Biochem. 1986;55:1151–1191. doi: 10.1146/annurev.bi.55.070186.005443. [DOI] [PubMed] [Google Scholar]
  8. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sarver N., Stollar V. Sindbis virus-induced cytopathic effect in clones of Aedes albopictus (Singh) cells. Virology. 1977 Jul 15;80(2):390–400. doi: 10.1016/s0042-6822(77)80014-7. [DOI] [PubMed] [Google Scholar]
  10. Shenk T. E., Koshelnyk K. A., Stollar V. Temperature-sensitive virus from Aedes albopictus cells chronically infected with Sindbis virus. J Virol. 1974 Feb;13(2):439–447. doi: 10.1128/jvi.13.2.439-447.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Stanners C. P., Francoeur A. M., Lam T. Analysis of VSV mutant with attenuated cytopathogenicity: mutation in viral function, P, for inhibition of protein synthesis. Cell. 1977 Jun;11(2):273–281. doi: 10.1016/0092-8674(77)90044-7. [DOI] [PubMed] [Google Scholar]
  12. Stevens T. M. Arbovirus replication in mosquito cell lines (Singh) grown in monolayer or suspension culture. Proc Soc Exp Biol Med. 1970 May;134(1):356–361. doi: 10.3181/00379727-134-34793. [DOI] [PubMed] [Google Scholar]
  13. Stollar V., Shenk T. E. Homologous viral interference in Aedes albopictus cultures chronically infected with Sindbis virus. J Virol. 1973 Apr;11(4):592–595. doi: 10.1128/jvi.11.4.592-595.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Storti R. V., Scott M. P., Rich A., Pardue M. L. Translational control of protein synthesis in response to heat shock in D. melanogaster cells. Cell. 1980 Dec;22(3):825–834. doi: 10.1016/0092-8674(80)90559-0. [DOI] [PubMed] [Google Scholar]
  15. Tatem J., Stollar V. Dominance of the CPE(+) phenotype in hybrid Aedes albopictus cells infected with Sindbis virus. Virus Res. 1986 Aug;5(2-3):121–130. doi: 10.1016/0168-1702(86)90012-2. [DOI] [PubMed] [Google Scholar]
  16. Tooker P., Kennedy S. I. Semliki Forest virus multiplication in clones of Aedes albopictus cells. J Virol. 1981 Feb;37(2):589–600. doi: 10.1128/jvi.37.2.589-600.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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