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. 1989 Mar;63(3):1101–1106. doi: 10.1128/jvi.63.3.1101-1106.1989

Molecular cloning of the complete genome of strain GDVII of Theiler's virus and production of infectious transcripts.

F Tangy 1, A McAllister 1, M Brahic 1
PMCID: PMC247804  PMID: 2536823

Abstract

We constructed a complete cDNA clone of strain GDVII of Theiler's virus in Bluescript plasmid. This recombinant plasmid, called pTMGDVII, was used to synthesize full-length RNA transcripts of the viral insert. This RNA was infectious for BHK cells. Virus R1-GDVII, obtained from transfected BHK cells, caused rapidly fatal encephalomyelitis in BALB/c mice. High amounts of viral antigens were present in neurons. No antigens were found in white matter. Therefore, the phenotype of R1-GDVII was indistinguishable from that of genuine GDVII Theiler's virus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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