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. 1989 Mar;63(3):1338–1344. doi: 10.1128/jvi.63.3.1338-1344.1989

Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine.

G D Westrop 1, K A Wareham 1, D M Evans 1, G Dunn 1, P D Minor 1, D I Magrath 1, F Taffs 1, S Marsden 1, M A Skinner 1, G C Schild 1, et al.
PMCID: PMC247831  PMID: 2536836

Abstract

The poliovirus type 3 Sabin oral poliovirus vaccine strain P3/Leon/12a1b differs in nucleotide sequence from its neurovirulent progenitor P3/Leon/37 by just 10 point mutations. The contribution of each mutation to the attenuation phenotype of the vaccine strain was determined by the construction of a series of recombinant viruses from infectious cDNA clones. The neurovirulence testing of recombinant viruses indicated that the attenuation phenotype is determined by just two point mutations: a C to U in the noncoding region at position 472 and a C to U at nucleotide 2034 which results in a serine-to-phenylalanine amino acid substitution in the structural protein VP3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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