Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1989 Mar;63(3):1489–1492. doi: 10.1128/jvi.63.3.1489-1492.1989

raf/myc-infected erythroid cells are restricted in their ability to terminally differentiate.

S P Klinken 1, U R Rapp 1, H C Morse 3rd 1
PMCID: PMC247858  PMID: 2915388

Abstract

A comparison was made of the in vitro erythroid colony-forming abilities of v-raf-, v-myc-, and v-raf/v-myc-containing retroviruses. In methylcellulose, v-raf efficiently produced colonies of well-differentiated hemoglobin-synthesizing erythroid cells, whereas v-raf/v-myc-infected erythroid cells were inhibited from terminally differentiating but retained the ability to replicate extensively. In contrast, v-myc was unable to stimulate the formation of erythroid colonies.

Full text

PDF
1489

Images in this article

1490Image
on p.1490

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson S. M., Klinken S. P., Hankins W. D. A murine recombinant retrovirus containing the src oncogene transforms erythroid precursor cells in vitro. Mol Cell Biol. 1985 Dec;5(12):3369–3375. doi: 10.1128/mcb.5.12.3369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baumbach W. R., Keath E. J., Cole M. D. A mouse c-myc retrovirus transforms established fibroblast lines in vitro and induces monocyte-macrophage tumors in vivo. J Virol. 1986 Aug;59(2):276–283. doi: 10.1128/jvi.59.2.276-283.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beug H., Hayman M. J. Temperature-sensitive mutants of avian erythroblastosis virus: surface expression of the erbB product correlates with transformation. Cell. 1984 Apr;36(4):963–972. doi: 10.1016/0092-8674(84)90046-1. [DOI] [PubMed] [Google Scholar]
  4. Brightman B. K., Pattengale P. K., Fan H. Generation and characterization of a recombinant Moloney murine leukemia virus containing the v-myc oncogene of avian MC29 virus: in vitro transformation and in vivo pathogenesis. J Virol. 1986 Oct;60(1):68–81. doi: 10.1128/jvi.60.1.68-81.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coppola J. A., Cole M. D. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment. Nature. 1986 Apr 24;320(6064):760–763. doi: 10.1038/320760a0. [DOI] [PubMed] [Google Scholar]
  6. Dmitrovsky E., Kuehl W. M., Hollis G. F., Kirsch I. R., Bender T. P., Segal S. Expression of a transfected human c-myc oncogene inhibits differentiation of a mouse erythroleukaemia cell line. Nature. 1986 Aug 21;322(6081):748–750. doi: 10.1038/322748a0. [DOI] [PubMed] [Google Scholar]
  7. Eliason J. F., Van Zant G., Goldwasser E. The relationship of hemoglobin synthesis to erythroid colony and burst formation. Blood. 1979 May;53(5):935–945. [PubMed] [Google Scholar]
  8. Frykberg L., Palmieri S., Beug H., Graf T., Hayman M. J., Vennström B. Transforming capacities of avian erythroblastosis virus mutants deleted in the erbA or erbB oncogenes. Cell. 1983 Jan;32(1):227–238. doi: 10.1016/0092-8674(83)90513-5. [DOI] [PubMed] [Google Scholar]
  9. Graf T., Ade N., Beug H. Temperature-sensitive mutant of avian erythroblastosis virus suggests a block of differentiation as mechanism of leukaemogenesis. Nature. 1978 Oct 12;275(5680):496–501. doi: 10.1038/275496a0. [DOI] [PubMed] [Google Scholar]
  10. Hankins W. D., Kost T. A., Koury M. J., Krantz S. B. Erythroid bursts produced by Friend leukaemia virus in vitro. Nature. 1978 Nov 30;276(5687):506–508. doi: 10.1038/276506a0. [DOI] [PubMed] [Google Scholar]
  11. Hankins W. D., Kost T. A., Pragnell I. B. The myeloproliferative sarcoma virus causes transformation or erythroid progenitor cells in vitro. Mol Cell Biol. 1982 Feb;2(2):138–146. doi: 10.1128/mcb.2.2.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hankins W. D., Scolnick E. M. Harvey and Kirsten sarcoma viruses promote the growth and differentiation of erythroid precursor cells in vitro. Cell. 1981 Oct;26(1 Pt 1):91–97. doi: 10.1016/0092-8674(81)90036-2. [DOI] [PubMed] [Google Scholar]
  13. Klinken S. P., Castilla M. J., Thorgeirsson S. S. Effect of inhibitors of ornithine decarboxylase on retrovirus induced transformation of murine erythroid precursors in vitro. Cancer Res. 1986 Dec;46(12 Pt 1):6246–6249. [PubMed] [Google Scholar]
  14. Klinken S. P. Erythroproliferation in vitro can be induced by abl, fes, src, ras, bas, raf, raf/myc, erb B and cbl oncogenes but not by myc, myb and fos. Oncogene Res. 1988 Sep;3(2):187–192. [PubMed] [Google Scholar]
  15. Klinken S. P., Nicola N. A., Johnson G. R. In vitro-derived leukemic erythroid cell lines induced by a raf- and myc-containing retrovirus differentiate in response to erythropoietin. Proc Natl Acad Sci U S A. 1988 Nov;85(22):8506–8510. doi: 10.1073/pnas.85.22.8506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lachman H. M., Cheng G. H., Skoultchi A. I. Transfection of mouse erythroleukemia cells with myc sequences changes the rate of induced commitment to differentiate. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6480–6484. doi: 10.1073/pnas.83.17.6480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morse H. C., 3rd, Hartley J. W., Fredrickson T. N., Yetter R. A., Majumdar C., Cleveland J. L., Rapp U. R. Recombinant murine retroviruses containing avian v-myc induce a wide spectrum of neoplasms in newborn mice. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6868–6872. doi: 10.1073/pnas.83.18.6868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rapp U. R., Cleveland J. L., Fredrickson T. N., Holmes K. L., Morse H. C., 3rd, Jansen H. W., Patschinsky T., Bister K. Rapid induction of hemopoietic neoplasms in newborn mice by a raf(mil)/myc recombinant murine retrovirus. J Virol. 1985 Jul;55(1):23–33. doi: 10.1128/jvi.55.1.23-33.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rapp U. R., Goldsborough M. D., Mark G. E., Bonner T. I., Groffen J., Reynolds F. H., Jr, Stephenson J. R. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4218–4222. doi: 10.1073/pnas.80.14.4218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Waneck G. L., Keyes L., Rosenberg N. Abelson virus drives the differentiation of Harvey virus-infected erythroid cells. Cell. 1986 Jan 31;44(2):337–344. doi: 10.1016/0092-8674(86)90768-3. [DOI] [PubMed] [Google Scholar]
  21. Waneck G. L., Rosenberg N. Abelson leukemia virus induces lymphoid and erythroid colonies in infected fetal cell cultures. Cell. 1981 Oct;26(1 Pt 1):79–89. doi: 10.1016/0092-8674(81)90035-0. [DOI] [PubMed] [Google Scholar]
  22. Zenke M., Kahn P., Disela C., Vennström B., Leutz A., Keegan K., Hayman M. J., Choi H. R., Yew N., Engel J. D. v-erbA specifically suppresses transcription of the avian erythrocyte anion transporter (band 3) gene. Cell. 1988 Jan 15;52(1):107–119. doi: 10.1016/0092-8674(88)90535-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES