Abstract
The epidermodysplasia verruciformis-associated human papillomavirus type 8 (HPV-8) poses a high risk for malignant conversion of skin lesions in patients with epidermodysplasia verruciformis. For seroepidemiological studies, the HPV-8 open reading frames for E1, E2, E4, E6, E7, and L1 were bacterially expressed as beta-galactosidase fusion proteins, which were purified by preparative gel electrophoresis. Cleavage with the protease FXa at the engineered recognition site separated the beta-galactosidase polypeptide part from the viral polypeptide. Western blot analysis of 445 serum samples from a randomly selected population with the entire L1 as antigen revealed HPV-8-specific immunoglobulin G antibodies in 20% of the samples. The percentage of positive sera did not significantly differ in different age groups. In some sera, we could also detect immunoglobulin M antibodies. The use of two shortened L1 polypeptides as antigen indicated that there are at least two reactive epitopes in the case of HPV-8 L1. Several sera contained antibodies to the early proteins E1, E2, E4, and E7. E1 and E7 were predominantly detected by sera which were negative for L1. In one case, we found antibodies to E6. Two of four sera of patients with epidermodysplasia verruciformis reacted with HPV-8 L1. The prevalence of anti-HPV-8-L1 antibodies in patients with malignant melanomas was comparable to that in the normal population (27.8%) but was significantly higher in patients with cervical cancer (37.5%), basaliomas (40%), and squamous cell skin carcinomas (72.7%) and in immunocompromised patients with Hodgkin's disease (47.7%).
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Selected References
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- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
- Cole S. T., Danos O. Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products. J Mol Biol. 1987 Feb 20;193(4):599–608. doi: 10.1016/0022-2836(87)90343-3. [DOI] [PubMed] [Google Scholar]
- Ellinger S., Glockshuber R., Jahn G., Plückthun A. Cleavage of procaryotically expressed human immunodeficiency virus fusion proteins by factor Xa and application in western blot (immunoblot) assays. J Clin Microbiol. 1989 May;27(5):971–976. doi: 10.1128/jcm.27.5.971-976.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fuchs P. G., Iftner T., Weninger J., Pfister H. Epidermodysplasia verruciformis-associated human papillomavirus 8: genomic sequence and comparative analysis. J Virol. 1986 May;58(2):626–634. doi: 10.1128/jvi.58.2.626-634.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fujikawa K., Legaz M. E., Davie E. W. Bovine factors X 1 and X 2 (Stuart factor). Isolation and characterization. Biochemistry. 1972 Dec 19;11(26):4882–4891. doi: 10.1021/bi00776a002. [DOI] [PubMed] [Google Scholar]
- Gassenmaier A., Fuchs P., Schell H., Pfister H. Papillomavirus DNA in warts of immunosuppressed renal allograft recipients. Arch Dermatol Res. 1986;278(3):219–223. doi: 10.1007/BF00412927. [DOI] [PubMed] [Google Scholar]
- Grimmel M., de Villiers E. M., Neumann C., Pawlita M., zur Hausen H. Characterization of a new human papillomavirus (HPV 41) from disseminated warts and detection of its DNA in some skin carcinomas. Int J Cancer. 1988 Jan 15;41(1):5–9. doi: 10.1002/ijc.2910410103. [DOI] [PubMed] [Google Scholar]
- Gross G., Ellinger K., Roussaki A., Fuchs P. G., Peter H. H., Pfister H. Epidermodysplasia verruciformis in a patient with Hodgkin's disease: characterization of a new papillomavirus type and interferon treatment. J Invest Dermatol. 1988 Jul;91(1):43–48. doi: 10.1111/1523-1747.ep12463287. [DOI] [PubMed] [Google Scholar]
- Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iftner T., Bierfelder S., Csapo Z., Pfister H. Involvement of human papillomavirus type 8 genes E6 and E7 in transformation and replication. J Virol. 1988 Oct;62(10):3655–3661. doi: 10.1128/jvi.62.10.3655-3661.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iftner T., Fuchs P. G., Pfister H. Two independently transforming functions of human papillomavirus 8. Curr Top Microbiol Immunol. 1989;144:167–173. doi: 10.1007/978-3-642-74578-2_20. [DOI] [PubMed] [Google Scholar]
- Jablonska S., Orth G. Epidermodysplasia verruciformis. Clin Dermatol. 1985 Oct-Dec;3(4):83–96. doi: 10.1016/0738-081x(85)90052-5. [DOI] [PubMed] [Google Scholar]
- Jenison S. A., Firzlaff J. M., Langenberg A., Galloway D. A. Identification of immunoreactive antigens of human papillomavirus type 6b by using Escherichia coli-expressed fusion proteins. J Virol. 1988 Jun;62(6):2115–2123. doi: 10.1128/jvi.62.6.2115-2123.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jenison S. A., Yu X. P., Valentine J. M., Galloway D. A. Human antibodies react with an epitope of the human papillomavirus type 6b L1 open reading frame which is distinct from the type-common epitope. J Virol. 1989 Feb;63(2):809–818. doi: 10.1128/jvi.63.2.809-818.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jochmus-Kudielka I., Schneider A., Braun R., Kimmig R., Koldovsky U., Schneweis K. E., Seedorf K., Gissmann L. Antibodies against the human papillomavirus type 16 early proteins in human sera: correlation of anti-E7 reactivity with cervical cancer. J Natl Cancer Inst. 1989 Nov 15;81(22):1698–1704. doi: 10.1093/jnci/81.22.1698. [DOI] [PubMed] [Google Scholar]
- Kawashima M., Favre M., Jablonska S., Obalek S., Orth G. Characterization of a new type of human papillomavirus (HPV) related to HPV5 from a case of actinic keratosis. Virology. 1986 Oct 30;154(2):389–394. doi: 10.1016/0042-6822(86)90464-2. [DOI] [PubMed] [Google Scholar]
- Koenen M., Rüther U., Müller-Hill B. Immunoenzymatic detection of expressed gene fragments cloned in the lac Z gene of E. coli. EMBO J. 1982;1(4):509–512. doi: 10.1002/j.1460-2075.1982.tb01199.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Li C. C., Shah K. V., Seth A., Gilden R. V. Identification of the human papillomavirus type 6b L1 open reading frame protein in condylomas and corresponding antibodies in human sera. J Virol. 1987 Sep;61(9):2684–2690. doi: 10.1128/jvi.61.9.2684-2690.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lutzner M. A., Orth G., Dutronquay V., Ducasse M. F., Kreis H., Crosnier J. Detection of human papillomavirus type 5 DNA in skin cancers of an immunosuppressed renal allograft recipient. Lancet. 1983 Aug 20;2(8347):422–424. doi: 10.1016/s0140-6736(83)90389-6. [DOI] [PubMed] [Google Scholar]
- Obałek S., Favre M., Jabłońska S., Szymańczyk J., Orth G. Human papillomavirus type 2-associated basal cell carcinoma in two immunosuppressed patients. Arch Dermatol. 1988 Jun;124(6):930–934. doi: 10.1001/archderm.1988.01670060076021. [DOI] [PubMed] [Google Scholar]
- Orth G., Jablonska S., Favre M., Croissant O., Jarzabek-Chorzelska M., Rzesa G. Characterization of two types of human papillomaviruses in lesions of epidermodysplasia verruciformis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1537–1541. doi: 10.1073/pnas.75.3.1537. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pass F., Reissig M., Shah K. V., Eisinger M., Orth G. Identification of an immunologically distinct papillomavirus from lesions of epidermodysplasia verruciformis. J Natl Cancer Inst. 1977 Oct;59(4):1107–1112. doi: 10.1093/jnci/59.4.1107. [DOI] [PubMed] [Google Scholar]
- Pfister H., Gassenmaier A., Fuchs P. G. Demonstration of human papillomavirus DNA in two keratoacanthomas. Arch Dermatol Res. 1986;278(3):243–246. doi: 10.1007/BF00412933. [DOI] [PubMed] [Google Scholar]
- Pfister H., Gassenmaier A., Nürnberger F., Stüttgen G. Human papilloma virus 5-DNA in a carcinoma of an epidermodysplasia verruciformis patient infected with various human papillomavirus types. Cancer Res. 1983 Mar;43(3):1436–1441. [PubMed] [Google Scholar]
- Pfister H., Nürnberger F., Gissmann L., zur Hausen H. Characterization of a human papillomavirus from epidermodysplasia verruciformis lesions of a patient from Upper-volta. Int J Cancer. 1981 May 15;27(5):645–650. doi: 10.1002/ijc.2910270511. [DOI] [PubMed] [Google Scholar]
- Pfister H., zur Hausen H. Seroepidemiological studies of human papilloma virus (HPV-1) infections. Int J Cancer. 1978 Feb 15;21(2):161–165. doi: 10.1002/ijc.2910210206. [DOI] [PubMed] [Google Scholar]
- Rüdlinger R., Smith I. W., Bunney M. H., Hunter J. A. Human papillomavirus infections in a group of renal transplant recipients. Br J Dermatol. 1986 Dec;115(6):681–692. doi: 10.1111/j.1365-2133.1986.tb06649.x. [DOI] [PubMed] [Google Scholar]
- Scheurlen W., Gissmann L., Gross G., zur Hausen H. Molecular cloning of two new HPV types (HPV 37 and HPV 38) from a keratoacanthoma and a malignant melanoma. Int J Cancer. 1986 Apr 15;37(4):505–510. doi: 10.1002/ijc.2910370406. [DOI] [PubMed] [Google Scholar]
- Steger G., Jarzabek-Chorzelska M., Jablonska S., Pfister H. Human papillomavirus 8-L1 immune serum: a new diagnostic possibility for Epidermodysplasia verruciformis-specific HPVs. J Invest Dermatol. 1988 Jul;91(1):76–81. doi: 10.1111/1523-1747.ep12463294. [DOI] [PubMed] [Google Scholar]