Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1990 Sep;64(9):4407–4413. doi: 10.1128/jvi.64.9.4407-4413.1990

AGM1+ spleen cells contain gamma interferon (IFN-gamma) gene transcripts in the early, sex-dependent production of IFN-gamma after picornavirus infection.

H I McFarland 1, N J Bigley 1
PMCID: PMC247909  PMID: 1974653

Abstract

Encephalomyocarditis D variant (EMCV-D)-infected spleen cell cultures prepared from diabetes-resistant ICR Swiss female mice produce more gamma interferon (IFN-gamma) activity over a 24-h period than do spleen cell cultures from diabetes-susceptible male mice of this strain. Pretreatment of mice with anti-asialo GM1 eliminates early in vitro IFN-gamma production from 4 to 16 h postinfection (p.i.) and reduces IFN-gamma production from 16 to 24 h p.i. In this study, depletion of spleen cells with anti-Thy-1 by panning greatly reduced IFN-gamma activity in EMCV-D-infected spleen cell cultures throughout a 24-h period. Populations of asialo GM1 (AGM1), L3T4, and Lyt-2-positive cells were isolated from cells harvested at 9 h p.i. from EMCV-D-infected spleen cell cultures by a modified panning technique on polystyrene microscope slides. By in situ hybridization with a [35S]dATP-labeled IFN-gamma cDNA probe, only the AGM1-bearing cells were found to contain detectable IFN-gamma gene transcripts. An AGM1+, Thy-1+ natural killer-like cell is the probable producer of the early, sex-dependent IFN-gamma activity in this system.

Full text

PDF
4407

Images in this article

4411Image
on p.4411

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bancroft G. J., Schreiber R. D., Bosma G. C., Bosma M. J., Unanue E. R. A T cell-independent mechanism of macrophage activation by interferon-gamma. J Immunol. 1987 Aug 15;139(4):1104–1107. [PubMed] [Google Scholar]
  2. Bigley N. J., Blay R. A., Smith R. A. Impaired cytokine response in male ICR Swiss mice after infection with D variant of encephalomyocarditis virus. Diabetes. 1987 Dec;36(12):1408–1413. doi: 10.2337/diab.36.12.1408. [DOI] [PubMed] [Google Scholar]
  3. Cohn Z. A. Activation of mononuclear phagocytes: fact, fancy, and future. J Immunol. 1978 Sep;121(3):813–816. [PubMed] [Google Scholar]
  4. Cosentino L. M., Cathcart M. K. A multi-step isolation scheme for obtaining CD16+ human natural killer cells. J Immunol Methods. 1987 Nov 5;103(2):195–204. doi: 10.1016/0022-1759(87)90290-0. [DOI] [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Giron D. J., Patterson R. R. Effect of steroid hormones on virus-induced diabetes mellitus. Infect Immun. 1982 Aug;37(2):820–822. doi: 10.1128/iai.37.2.820-822.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Giron D. J., Pindak F. F. Propagation of MM virus in L cells. Appl Microbiol. 1969 Jun;17(6):811–814. doi: 10.1128/am.17.6.811-814.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hardy K. J., Peterlin B. M., Atchison R. E., Stobo J. D. Regulation of expression of the human interferon gamma gene. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8173–8177. doi: 10.1073/pnas.82.23.8173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heckford S. E., Gelmann E. P., Agnor C. L., Jacobson S., Zinn S., Matis L. A. Distinct signals are required for proliferation and lymphokine gene expression in murine T cell clones. J Immunol. 1986 Dec 1;137(11):3652–3663. [PubMed] [Google Scholar]
  10. Herberman R. B., Nunn M. E., Holden H. T. Low density of Thy 1 antigen on mouse effector cells mediating natural cytotoxicity against tumor cells. J Immunol. 1978 Jul;121(1):304–309. [PubMed] [Google Scholar]
  11. Herold K. C., Lancki D. W., Dunn D. E., Arai K., Fitch F. W. Activation of lymphokine genes during stimulation of cloned T cells. Eur J Immunol. 1986 Dec;16(12):1533–1538. doi: 10.1002/eji.1830161211. [DOI] [PubMed] [Google Scholar]
  12. Kasaian M. T., Biron C. A. The activation of IL-2 transcription in L3T4+ and Lyt-2+ lymphocytes during virus infection in vivo. J Immunol. 1989 Feb 15;142(4):1287–1292. [PubMed] [Google Scholar]
  13. Knobloch C., Dennert G. Asialo-GM1-positive T killer cells are generated in F1 mice injected with parental spleen cells. J Immunol. 1988 Feb 1;140(3):744–749. [PubMed] [Google Scholar]
  14. Lawrence J. B., Singer R. H. Quantitative analysis of in situ hybridization methods for the detection of actin gene expression. Nucleic Acids Res. 1985 Mar 11;13(5):1777–1799. doi: 10.1093/nar/13.5.1777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Le J., Prensky W., Yip Y. K., Chang Z., Hoffman T., Stevenson H. C., Balazs I., Sadlik J. R., Vilcek J. Activation of human monocyte cytotoxicity by natural and recombinant immune interferon. J Immunol. 1983 Dec;131(6):2821–2826. [PubMed] [Google Scholar]
  16. McFarland H. I., Bigley N. J. Sex-dependent, early cytokine production by NK-like spleen cells following infection with the D variant of encephalomyocarditis virus (EMCV-D). Viral Immunol. 1989 Fall;2(3):205–214. doi: 10.1089/vim.1989.2.205. [DOI] [PubMed] [Google Scholar]
  17. Nagler A., Lanier L. L., Cwirla S., Phillips J. H. Comparative studies of human FcRIII-positive and negative natural killer cells. J Immunol. 1989 Nov 15;143(10):3183–3191. [PubMed] [Google Scholar]
  18. Roberts W. K., Vasil A. Evidence for the identity of murine gamma interferon and macrophage activating factor. J Interferon Res. 1982;2(4):519–532. doi: 10.1089/jir.1982.2.519. [DOI] [PubMed] [Google Scholar]
  19. Soldateschi D., Boraschi D., Tagliabue A. Natural antiviral activity of mouse macrophages against encephalomyocarditis virus. Antiviral Res. 1985 Aug;5(4):217–227. doi: 10.1016/0166-3542(85)90026-9. [DOI] [PubMed] [Google Scholar]
  20. Suttles J., Schwarting G. A., Stout R. D. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes. J Immunol. 1986 Mar 1;136(5):1586–1591. [PubMed] [Google Scholar]
  21. Torres B. A., Yamamoto J. K., Johnson H. M. Cellular regulation of gamma interferon production: Lyt phenotype of the suppressor cell. Infect Immun. 1982 Mar;35(3):770–776. doi: 10.1128/iai.35.3.770-776.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Weiss A., Wiskocil R. L., Stobo J. D. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level. J Immunol. 1984 Jul;133(1):123–128. [PubMed] [Google Scholar]
  23. Wiskocil R., Weiss A., Imboden J., Kamin-Lewis R., Stobo J. Activation of a human T cell line: a two-stimulus requirement in the pretranslational events involved in the coordinate expression of interleukin 2 and gamma-interferon genes. J Immunol. 1985 Mar;134(3):1599–1603. [PubMed] [Google Scholar]
  24. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yoon J. W., McClintock P. R., Onodera T., Notkins A. L. Virus-induced diabetes mellitus. XVIII. Inhibition by a nondiabetogenic variant of encephalomyocarditis virus. J Exp Med. 1980 Oct 1;152(4):878–892. doi: 10.1084/jem.152.4.878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Young H. A., Ortaldo J. R. One-signal requirement for interferon-gamma production by human large granular lymphocytes. J Immunol. 1987 Aug 1;139(3):724–727. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES