Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1970 Jul;103(1):16–19. doi: 10.1128/jb.103.1.16-19.1970

Regulation of Aromatic Amino Acid Biosynthesis in Phenazine-Producing Strains of Pseudomonas

Mark E Levitch 1
PMCID: PMC248032  PMID: 5423368

Abstract

Regulation of 3-deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthetase was studied in eight strains of Pseudomonas which synthesize phenazine compounds. Repression studies with individual aromatic amino acids led to the finding that enzyme synthesis was repressed in only one strain, P. aureofaciens B1543p, and by only one amino acid, l-tyrosine. Feedback inhibition by the aromatic amino acids varied from strain to strain in terms of the type of inhibitory control, and the particular acid or acids which inhibited. Prephenate and chorismate, as well as a number of naturally occurring phenazine compounds, inhibited the DAHP synthetase activity to varying degrees.

Full text

PDF
16

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COTTON R. G., GIBSON F. THE BIOSYNTHESIS OF PHENYLALANINE AND TYROSINE; ENZYMES CONVERTING CHORISMIC ACID INTO PREPHENIC ACID AND THEIR RELATIONSHIPS TO PREPHENATE DEHYDRATASE AND PREPHENATE DEHYDROGENASE. Biochim Biophys Acta. 1965 Apr 12;100:76–88. doi: 10.1016/0304-4165(65)90429-0. [DOI] [PubMed] [Google Scholar]
  2. Chang P. C., Blackwood A. C. Simultaneous biosynthesis of pyocyanine, phenazine-1-carboxylic acid, and oxychloroaphine from labelled substrates by Pseudomonas aeruginosa Mac 436. Can J Biochem. 1968 Aug;46(8):925–929. doi: 10.1139/o68-138. [DOI] [PubMed] [Google Scholar]
  3. Cotton R. G., Gibson F. The biosynthesis of phenylalanine and tyrosine in the pea (Pisum sativum): chorismate mutase. Biochim Biophys Acta. 1968 Feb 1;156(1):187–189. doi: 10.1016/0304-4165(68)90118-9. [DOI] [PubMed] [Google Scholar]
  4. DATTA P., GEST H. CONTROL OF ENZYME ACTIVITY BY CONCERTED FEEDBACK INHIBITION. Proc Natl Acad Sci U S A. 1964 Oct;52:1004–1009. doi: 10.1073/pnas.52.4.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVIS B. D. Aromatic biosynthesis. I. The role of shikimic acid. J Biol Chem. 1951 Jul;191(1):315–325. [PubMed] [Google Scholar]
  6. DeMoss J. A., Wegman J. An enzyme aggregate in the tryptophan pathway of Neurospora crassa. Proc Natl Acad Sci U S A. 1965 Jul;54(1):241–247. doi: 10.1073/pnas.54.1.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Doy C. H. Aromatic biosynthesis in yeast. II. Feedback inhibition and repression of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase. Biochim Biophys Acta. 1968 Jan 8;151(1):293–295. doi: 10.1016/0005-2744(68)90190-3. [DOI] [PubMed] [Google Scholar]
  8. Doy C. H., Brown K. D. Control of aromatic biosynthesis: the multiplicity of 7-phospho-2-oxo-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating) in Escherichia coli W. Biochim Biophys Acta. 1965 Jul 8;104(2):377–389. doi: 10.1016/0304-4165(65)90343-0. [DOI] [PubMed] [Google Scholar]
  9. GERBER N. N., LECHEVALIER M. P. PHENAZINES AND PHENOXAZINONES FROM WAKSMANIA AERATA SP. NOV. AND PSEUDOMONAS IODINA. Biochemistry. 1964 Apr;3:598–602. doi: 10.1021/bi00892a022. [DOI] [PubMed] [Google Scholar]
  10. Gibson F. Chorismic acid: purification and some chemical and physical studies. Biochem J. 1964 Feb;90(2):256–261. doi: 10.1042/bj0900256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ito J., Crawford I. P. Regulation of the enzymes of the tryptophan pathway in Escherichia coli. Genetics. 1965 Dec;52(6):1303–1316. doi: 10.1093/genetics/52.6.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JENSEN R. A., NESTER E. W. THE REGULATORY SIGNIFICANCE OF INTERMEDIARY METABOLITES: CONTROL OF AROMATIC ACID BIOSYNTHESIS BY FEEDBACK INHIBITION IN BACILLUS SUBTILIS. J Mol Biol. 1965 Jun;12:468–481. doi: 10.1016/s0022-2836(65)80270-4. [DOI] [PubMed] [Google Scholar]
  13. Jensen R. A., Nasser D. S., Nester E. W. Comparative control of a branch-point enzyme in microorganisms. J Bacteriol. 1967 Nov;94(5):1582–1593. doi: 10.1128/jb.94.5.1582-1593.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jensen R. A., Nester E. W. Regulatory enzymes of aromatic amino acid biosynthesis in Bacillus subtilis. I. Purification and properties of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase. J Biol Chem. 1966 Jul 25;241(14):3365–3372. [PubMed] [Google Scholar]
  15. KLUYVER A. J. Pseudomonas aureofaciens nov. spec. and its pigments. J Bacteriol. 1956 Sep;72(3):406–411. doi: 10.1128/jb.72.3.406-411.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LEVITCH M. E., STADTMAN E. R. STUDY OF THE BIOSYNTHESIS OF PHENAZINE-1-CARBOXYLIC ACID. Arch Biochem Biophys. 1964 Jul 20;106:194–199. doi: 10.1016/0003-9861(64)90175-4. [DOI] [PubMed] [Google Scholar]
  17. Levitch M. E., Rietz P. The isolation and characterization of 2-hydroxyphenazine from Pseudomonas aureofaciens. Biochemistry. 1966 Feb;5(2):689–692. doi: 10.1021/bi00866a040. [DOI] [PubMed] [Google Scholar]
  18. SMITH L. C., RAVEL J. M., LAX S. R., SHIVE W. The control of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthesis by phenylalanine and tyrosine. J Biol Chem. 1962 Nov;237:3566–3570. [PubMed] [Google Scholar]
  19. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  20. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES