Abstract
The inorganic and polyphosphate pools of Saccharomyces mellis, grown in a medium containing excess phosphate, remain associated with the cells when the cells are suspended in a saline medium. If the cells are incubated in a medium containing 2 m KCl, the cells are altered in some manner which permits most of the orthophosphate and approximately one-third of the polyphosphate to be subsequently eluted by osmotic shock. At lower salt concentrations, β-mercaptoethanol enhances this salt effect but is inactive by itself in this respect. At equivalent ionic strengths, the sodium salt of ethylenediaminetetraacetic acid behaves exactly like KCl or any other monovalent ionic compound in altering the cell to susceptibility to osmotic shock. No special effect of this anion at either high or low concentration could be detected. Resting cells are refractory to being altered in this manner by salts if an energy source, such as glucose, is included in the reaction mixture. Cells which are depleted of phosphate reserves will immediately incorporate phosphate when suspended in a medium containing inorganic phosphate and an energy source. These cells exhibit the phenomenon of “überkompensation.” In resting cells, the inclusion of KCl in the reaction mixture prevents the conversion of orthophosphate into polyphosphate and, also, gradually decreases the ability of the organism even to assimilate orthophosphate. This effect is reversible, however, since the cells will incorporate phosphate in a normal manner if the cells are transferred to a non-salinized medium, or if a nitrogen source is included in the salinized reaction mixture so that the cells are now in a medium adequate for growth.
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Selected References
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- Blum J. J. Phosphate uptake by phosphate-starved Euglena. J Gen Physiol. 1966 Jul;49(6):1125–1137. doi: 10.1085/jgp.0491125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Borst Pauwels G. W. A study of the release of phosphate and arsenate from yeast. J Cell Physiol. 1967 Apr;69(2):241–246. doi: 10.1002/jcp.1040690214. [DOI] [PubMed] [Google Scholar]
- Harold F. M. Inorganic polyphosphates in biology: structure, metabolism, and function. Bacteriol Rev. 1966 Dec;30(4):772–794. doi: 10.1128/br.30.4.772-794.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heppel L. A. Selective release of enzymes from bacteria. Science. 1967 Jun 16;156(3781):1451–1455. doi: 10.1126/science.156.3781.1451. [DOI] [PubMed] [Google Scholar]
- Indge K. J. Polyphosphates of the yeast cell vacuole. J Gen Microbiol. 1968 May;51(3):447–455. doi: 10.1099/00221287-51-3-447. [DOI] [PubMed] [Google Scholar]
- Jungnickel F. Vergleich des Einflusses der Veratmung endogener und exogener Substrate auf Polyphosphatbildung und Kaliumaufnahme bei phosphatverarmten Zellen von Candida utilis. Arch Mikrobiol. 1966 Nov 11;55(2):175–186. [PubMed] [Google Scholar]
- Kulaev I. S., Krasheninnikov I. A., Kokurina N. K. O lokalizatsii neorganicheskikh polifosfatov i nukleotidov v mitselii Neurospora crassa. Biokhimiia. 1966 Jul-Aug;31(4):850–859. [PubMed] [Google Scholar]
- Kulaev I. S., Shimona O., Bobyk M. A. O biosinteze neorganicheskikh polifosfatov u Neurospora crassa. Biokhimiia. 1968 May-Jun;33(3):419–434. [PubMed] [Google Scholar]
- Kulaev I. S., Vagabov V. M. Vliianie uslovii vyrashchivaniia na obmen neorganicheskikh polifosfatov i nekotorykh drugikh fosfornykh soedinenii u Scenedesmus obliquus. Biokhimiia. 1967 Mar-Apr;32(2):253–260. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Leive L. Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate. J Biol Chem. 1968 May 10;243(9):2373–2380. [PubMed] [Google Scholar]
- Munk N., Rosenberg H. On the deposition and utilization of inorganic pyrophosphate in Tetrahymena pyriformis. Biochim Biophys Acta. 1969 May 6;177(3):629–640. doi: 10.1016/0304-4165(69)90329-8. [DOI] [PubMed] [Google Scholar]
- ROTHSTEIN A. Role of the cell membrane in the metabolism of inorganic electrolytes by microorganisms. Bacteriol Rev. 1959 Dec;23(4):175–201. doi: 10.1128/br.23.4.175-201.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Souzu H. Location of polyphosphate and polyphosphatase in yeast cells and damage to the protoplasmic membrane of the cell by freeze-thawing. Arch Biochem Biophys. 1967 May;120(2):344–351. doi: 10.1016/0003-9861(67)90249-4. [DOI] [PubMed] [Google Scholar]
- VAN STEVENINCK, BOOIJ H. L. THE ROLE OF POLYPHOSPHATES IN THE TRANSPORT MECHANISM OF GLUCOSE IN YEAST CELLS. J Gen Physiol. 1964 Sep;48:43–60. doi: 10.1085/jgp.48.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIMBERG R., ORTON W. L. EVIDENCE FOR AN EXOCELLULAR SITE FOR THE ACID PHOSPHATASE OF SACCHAROMYCES MELLIS. J Bacteriol. 1964 Dec;88:1743–1754. doi: 10.1128/jb.88.6.1743-1754.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIMBERG R., ORTON W. L. REPRESSIBLE ACID PHOSPHOMONOESTERASE AND CONSTITUTIVE PYROPHOSPHATASE OF SACCHAROMYCES MELLIS. J Bacteriol. 1963 Oct;86:805–813. doi: 10.1128/jb.86.4.805-813.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIMBERG R., ORTON W. L. SYNTHESIS AND BREAKDOWN OF THE POLYPHOSPHATE FRACTION AND ACID PHOSPHOMONOESTERASE OF SACCHAROMYCES MELLIS AND THEIR LOCATIONS IN THE CELL. J Bacteriol. 1965 Mar;89:740–747. doi: 10.1128/jb.89.3.740-747.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weimberg R., Orton W. L. Elution of Acid Phosphatase from the Cell Surface of Saccharomyces mellis by Potassium Chloride. J Bacteriol. 1965 Jul;90(1):82–94. doi: 10.1128/jb.90.1.82-94.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]