Abstract
The utilization of d-mannitol, d-arabitol, and d-sorbitol by Rhizobium meliloti was studied in extracts from mannitol-grown cells. Two different polyol dehydrogenases were induced by any of these polyols: (i) a nicotinamide adenine dinucleotide (NAD)-arabitol dehydrogenase and (ii) a NAD-sorbitol dehydrogenase, whereas polyol phosphate dehydrogenases were absent. d-Arabitol dehydrogenase was observed to act on both d-arabitol and d-mannitol, but d-sorbitol dehydrogenase acted specifically on d-sorbitol. d-Arabitol was oxidized to d-xylulose, d-mannitol and d-sorbitol were oxidized to d-fructose. An adenosine triphosphate-linked hexokinase which acts on d-fructose and absence of hexose isomerase were also detected in this organism.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DISCHE Z., BORENFREUND E. A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem. 1951 Oct;192(2):583–587. [PubMed] [Google Scholar]
- SCOLNICK E. M., LIN E. C. Parallel induction of D-arabitol and D-sorbitol dehydrogenases. J Bacteriol. 1962 Oct;84:631–637. doi: 10.1128/jb.84.4.631-637.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOLFF J. B., KAPLAN N. O. D-Mannitol 1-phosphate dehydrogenase from Escherichia coli. J Biol Chem. 1956 Feb;218(2):849–869. [PubMed] [Google Scholar]
- WU R., RACKER E. Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells. J Biol Chem. 1959 May;234(5):1029–1035. [PubMed] [Google Scholar]
- Wilson P. W. RESPIRATORY ENZYME SYSTEMS IN SYMBIOTIC NITROGEN FIXATION I. : The "Resting Cell" Technique as a Method for Study of Bacterial Metabolism. J Bacteriol. 1938 Jun;35(6):601–623. doi: 10.1128/jb.35.6.601-623.1938. [DOI] [PMC free article] [PubMed] [Google Scholar]