Abstract
The fine structure of lipopolysaccharide (LPS), isolated from an avian strain of Escherichia coli O18, was examined by electron microscopy. In positively stained preparations, ribbonlike structures with frequent branching were observed as previously reported (4). Two densely stained parallel lines were occasionally seen associated with a ribbon. When negative staining was employed, the LPS appeared as a branching ribbon with one central and two lateral zones divided by two relatively dense parallel lines running the complete length of the ribbon. The lateral zones were probably due to the O-antigenic side chains of the LPS. This interpretation was supported by the fact that the electron microscopic structure of the LPS from two rough strains, E. coli K-12 Gal 23 and Salmonella tuphimurium TV119 RII, both lacking the O-specific side chains, did not possess the outer lateral zones.
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- BANGHAM A. D., HORNE R. W. NEGATIVE STAINING OF PHOSPHOLIPIDS AND THEIR STRUCTURAL MODIFICATION BY SURFACE-ACTIVE AGENTS AS OBSERVED IN THE ELECTRON MICROSCOPE. J Mol Biol. 1964 May;8:660–668. doi: 10.1016/s0022-2836(64)80115-7. [DOI] [PubMed] [Google Scholar]
- BLADEN H. A., MERGENHAGEN S. E. ULTRASTRUCTURE OF VEILLONELLA AND MORPHOLOGICAL CORRELATION OF AN OUTER MEMBRANE WITH PARTICLES ASSOCIATED WITH ENDOTOXIC ACTIVITY. J Bacteriol. 1964 Nov;88:1482–1492. doi: 10.1128/jb.88.5.1482-1492.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beer H., Braude A. I., Brinton C. C., Jr A study of particle sizes, shapes and toxicities present in a boivin-type endotoxic preparation. Ann N Y Acad Sci. 1966 Jun 30;133(2):450–475. doi: 10.1111/j.1749-6632.1966.tb52383.x. [DOI] [PubMed] [Google Scholar]
- LUCY J. A., GLAUERT A. M. STRUCTURE AND ASSEMBLY OF MACROMOLECULAR LIPID COMPLEXES COMPOSED OF GLOBULAR MICELLES. J Mol Biol. 1964 May;8:727–748. doi: 10.1016/s0022-2836(64)80121-2. [DOI] [PubMed] [Google Scholar]
- MILNER K. C., ANACKER R. L., FUKUSHI K., HASKINS W. T., LANDY M., MALMGREN B., RIBI E. SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. III. STRUCTURE AND BIOLOGICAL PROPERTIES OF SURFACE ANTIGENS FROM GRAM-NEGATIVE BACTERIA. Bacteriol Rev. 1963 Dec;27:352–368. doi: 10.1128/br.27.4.352-368.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McIntire F. C., Sievert H. W., Barlow G. H., Finley R. A., Lee A. Y. Chemical, physical, biological properties of a lipopolysaccharide from Escherichia coli K-235. Biochemistry. 1967 Aug;6(8):2363–2372. doi: 10.1021/bi00860a011. [DOI] [PubMed] [Google Scholar]
- Rapin A. M., Mayer H. Complex polysaccharides in different strains of Escherichia coli K-12. Ann N Y Acad Sci. 1966 Jun 30;133(2):425–437. doi: 10.1111/j.1749-6632.1966.tb52381.x. [DOI] [PubMed] [Google Scholar]
- Rothfield L., Horne R. W. Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic and monolayer studies. J Bacteriol. 1967 May;93(5):1705–1721. doi: 10.1128/jb.93.5.1705-1721.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shands J. W., Jr, Graham J. A., Nath K. The morphologic structure of isolated bacterial lipopolysaccharide. J Mol Biol. 1967 Apr 14;25(1):15–21. doi: 10.1016/0022-2836(67)90275-6. [DOI] [PubMed] [Google Scholar]
- WEIDEL W., FRANK H., MARTIN H. H. The rigid layer of the cell wall of Escherichia coli strain B. J Gen Microbiol. 1960 Feb;22:158–166. doi: 10.1099/00221287-22-1-158. [DOI] [PubMed] [Google Scholar]
- Work E., Knox K. W., Vesk M. The chemistry and electron microscopy of an extracellular lipopolysaccharide from Escherichia coli. Ann N Y Acad Sci. 1966 Jun 30;133(2):438–449. doi: 10.1111/j.1749-6632.1966.tb52382.x. [DOI] [PubMed] [Google Scholar]