Abstract
Induction of kynureninase in a homogeneous population of mycelia in balanced growth was studied. Induced synthesis of enzyme was apparent 10 min after addition of inducers (tryptophan, kynurenine, and N-formylkynurenine). Experiments with cycloheximide-inhibited cells indicated that initiation of message transcription occurred about 4 min after addition of inducers. Lifetime of the message in the absence of inducer was of the order of 5 min. Experiments with mutants showed that N-formylkynurenine was the probable inducer of physiological importance.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bates W. K., Hedman S. C., Woodward D. O. Comparative inductive responses of two beta-galactosidases of Neurospora. J Bacteriol. 1967 May;93(5):1631–1637. doi: 10.1128/jb.93.5.1631-1637.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEMOSS J. A. Studies on the mechanism of the tryptophan synthetase reaction. Biochim Biophys Acta. 1962 Aug 13;62:279–293. doi: 10.1016/0006-3002(62)90041-0. [DOI] [PubMed] [Google Scholar]
- GROSS S. R., GAFFORD R. D., TATUM E. L. The metabolism of protocatechuic acid by Neurospora. J Biol Chem. 1956 Apr;219(2):781–796. [PubMed] [Google Scholar]
- Haskins F. A., Mitchell H. K. Evidence for a Tryptophane Cycle in Neurospora. Proc Natl Acad Sci U S A. 1949 Sep;35(9):500–506. doi: 10.1073/pnas.35.9.500. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JAKOBY W. B., BONNER D. M. Kynureninase from Neurospora: purification and properties. J Biol Chem. 1953 Dec;205(2):699–707. [PubMed] [Google Scholar]
- MATCHETT W. H., DEMOSS J. A. Direct evidence for a trytophan-anthranilic acid cycle in Neurospora. Biochim Biophys Acta. 1963 Jun 4;71:632–642. doi: 10.1016/0006-3002(63)91136-3. [DOI] [PubMed] [Google Scholar]
- PARTRIDGE C. W. H., BONNER D. M., YANOFSKY C. A quantitative study of the relationship between tryptophan and niacin in Neurospora. J Biol Chem. 1952 Jan;194(1):269–278. [PubMed] [Google Scholar]
- Turner J. R., Matchett W. H. Alteration of tryptophan-mediated regulation in Neurospora crassa by indoleglycerol phosphate. J Bacteriol. 1968 May;95(5):1608–1614. doi: 10.1128/jb.95.5.1608-1614.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turner J. R., Terry K., Matchett W. H. Temporal separation of transcription and translation in Neurospora. J Bacteriol. 1970 Aug;103(2):370–374. doi: 10.1128/jb.103.2.370-374.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WAINWRIGHT S. D., BONNER D. M. On the induced synthesis of an enzyme required for biosynthesis of an essential metabolite: induced kynureninase synthesis in Neurospora crassa. Can J Biochem Physiol. 1959 Jun;37(6):741–750. [PubMed] [Google Scholar]