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. 1973;49(4):327–332.

Use of the single radial diffusion technique for influenza antibody surveys

Pratima Chakraverty, Marguerite S Pereira, G C Schild
PMCID: PMC2480947  PMID: 4212505

Abstract

A survey of antibody to the influenza A/Hong Kong/1/68 (H3N2) and the variant A/England/42/72 (H3N2) was carried out by two methods, haemagglutination-inhibition (HI) and single radial diffusion (SRD). A comparison of the sensitivity of the two tests indicated that the proportion of sera that produced a zone of 2.5 mm or larger in the SRD tests was equivalent to the proportion that gave HI titres of 1: 40 or more. The test was carried out on serum samples collected from various regions of England. The results showed that approximately 70% had antibody to the A/HK/1/68 virus and only 35% had antibody to A/England/42/72. The SRD test was shown to have several practical advantages for seroepidemiological studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Kilbourne E. D. Future influenza vaccines and the use of genetic recombinants. Bull World Health Organ. 1969;41(3):643–645. [PMC free article] [PubMed] [Google Scholar]
  2. Miller D. L., Lee J. A. Influenza in Britain 1967-68. J Hyg (Lond) 1969 Sep;67(3):559–572. doi: 10.1017/s0022172400042005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Miller D. L., Reid D., Daimond J. R., Pereira M. S., Chakraverty P. Hong Kong influenza in the Royal Air Force 1968-70. J Hyg (Lond) 1973 Sep;71(3):535–547. doi: 10.1017/s0022172400046520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Pereira M. S., Miller D. L., Clarke M. Serological evidence of infection with A2 Hong Kong influenza virus during the epidemic in Britain 1968-9. Br Med J. 1969 Dec 27;4(5686):815–815. [PMC free article] [PubMed] [Google Scholar]
  5. Schild G. C., Henry-Aymard M., Pereira H. G. A quantitative, single-radial-diffusion test for immunological studies with influenza virus. J Gen Virol. 1972 Aug;16(2):231–236. doi: 10.1099/0022-1317-16-2-231. [DOI] [PubMed] [Google Scholar]
  6. Schild G. C., Pereira H. G. Characterization of the ribonucleoprotein and neuraminidase of influenza A viruses by immunodiffusion. J Gen Virol. 1969 Apr;4(3):355–363. doi: 10.1099/0022-1317-4-3-355. [DOI] [PubMed] [Google Scholar]
  7. Skehel J. J., Schild G. C. The polypeptide composition of influenza A viruses. Virology. 1971 May;44(2):396–408. doi: 10.1016/0042-6822(71)90270-4. [DOI] [PubMed] [Google Scholar]

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