Abstract
Deoxyadenosine (AdR) and adenosine (AR) enhance the incorporation of thymidine (TdR) into bacterial deoxyribonucleic acid (DNA) by the inhibition of TdR phosphorolysis in vivo. Neither of the purine nucleosides has an effect on the reaction catalyzed by TdR phosphorylase in vitro. AdR induces TdR phosphorylase and both purine nucleosides induce purine nucleoside phosphorylase. AR can stimulate uptake of more TdR into bacterial DNA than AdR.
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Selected References
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- Ahmad S. I., Pritchard R. H. A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli. Mol Gen Genet. 1969 Aug 15;104(4):351–359. doi: 10.1007/BF00334234. [DOI] [PubMed] [Google Scholar]
- BECK W. S., LEVIN M. Purification, kinetics, and repression control of bacterial trans-N-deoxyribosylase. J Biol Chem. 1963 Feb;238:702–709. [PubMed] [Google Scholar]
- BODMER W. F., GRETHER S. UPTAKE AND INCORPORATION OF THYMINE, THYMIDINE, URACIL, URIDINE, AND 5-FLUOROURACIL INTO THE NUCLEIC ACIDS OF BACILLUS SUBTILIS. J Bacteriol. 1965 Apr;89:1011–1014. doi: 10.1128/jb.89.4.1011-1014.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Budman D. R., Pardee A. B. Thymidine and thymine incorporation into deoxyribonucleic acid: inhibition and repression by uridine of thymidine phosphorylase of Escherichia coli. J Bacteriol. 1967 Nov;94(5):1546–1550. doi: 10.1128/jb.94.5.1546-1550.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doskocil J., Paces V. The effect of nucleosides on the phosphorolysis of thymidine by normal and phage-infected cells of E. coli. Biochem Biophys Res Commun. 1968 Jan 25;30(2):153–158. doi: 10.1016/0006-291x(68)90463-4. [DOI] [PubMed] [Google Scholar]
- Fangman W. L., Novick A. Mutant bacteria showing efficient utilization of thymidine. J Bacteriol. 1966 Jun;91(6):2390–2391. doi: 10.1128/jb.91.6.2390-2391.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fangman W. L. Specificity and efficiency of thymidine incorporation in Escherichia coli lacking thymidine phosphorylase. J Bacteriol. 1969 Sep;99(3):681–687. doi: 10.1128/jb.99.3.681-687.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lomax M. S., Greenberg G. R. Characteristics of the deo operon: role in thymine utilization and sensitivity to deoxyribonucleosides. J Bacteriol. 1968 Aug;96(2):501–514. doi: 10.1128/jb.96.2.501-514.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANS R. J., KOCH A. L. Metabolism of adenosine and deoxyadenosine by growing cultures of Escherichia coli. J Biol Chem. 1960 Feb;235:450–456. [PubMed] [Google Scholar]
- Munch-Petersen A. Thymidine breakdown and thymine uptake in different mutants of Escherichia coli. Biochim Biophys Acta. 1967 Jun 20;142(1):228–237. doi: 10.1016/0005-2787(67)90530-8. [DOI] [PubMed] [Google Scholar]
- RACHMELER M., GERHART J., ROSNER J. Limited thymidine uptake in Escherichia coli due to an inducible thymidine phosphorylase. Biochim Biophys Acta. 1961 Apr 29;49:222–225. doi: 10.1016/0006-3002(61)90888-5. [DOI] [PubMed] [Google Scholar]
- RAZZELL W. E., CASSHYAP P. SUBSTRATE SPECIFICITY AND INDUCTION OF THYMIDINE PHOSPHORYLASE IN ESCHERICHIA COLI. J Biol Chem. 1964 Jun;239:1789–1793. [PubMed] [Google Scholar]
- Rosner A., Yagil E. Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants. Mol Gen Genet. 1970;106(3):254–262. doi: 10.1007/BF00340384. [DOI] [PubMed] [Google Scholar]
- TSUBOI K. K., HUDSON P. B. Enzymes of the human erythrocyte. I. Purine nucleoside phosphorylase; isolation procedure. J Biol Chem. 1957 Feb;224(2):879–887. [PubMed] [Google Scholar]