Abstract
The adequate characterization of type A influenzaviruses requires the identification not only of the haemagglutinin but also of the neuraminidase. The neuraminidase-inhibition test, as performed in the two WHO international reference centres for influenza, is described and its use in other laboratories—particularly those collaborating in the WHO programme—is recommended.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADA G. L., LIND P. E. AN IMMUNOLOGICAL STUDY OF AVIAN, VIRAL AND BACTERIAL NEURAMINIDASE BASED ON SPECIFIC INHIBITION OF ENZYME BY ANTIBODY. J Gen Microbiol. 1963 Aug;32:225–233. doi: 10.1099/00221287-32-2-225. [DOI] [PubMed] [Google Scholar]
- AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dimmock N. J. Dependence of the activity of an influenza virus neuraminidase upon Ca2+. J Gen Virol. 1971 Dec;13(3):481–483. doi: 10.1099/0022-1317-13-3-481. [DOI] [PubMed] [Google Scholar]
- WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
- Webster R. G., Laver W. G. Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. J Immunol. 1967 Jul;99(1):49–55. [PubMed] [Google Scholar]