Abstract
Decay of the reduced nicotinamide adenine dinucleotide oxidase of Bacillus megaterium KM membranes was prevented by storage in 10% (v/v) glycerol or 0.4% bovine serum albumin. Differential rates of solubilization of components of the oxidase system by 0.4% deoxycholate was demonstrable at 4 C. The amount of Mg++ necessary for maximal oxidase reactivation increased with increasing amounts of deoxycholate-inactivated oxidase. Mg++ activation of deoxycholate-inactivated oxidase was partially temperature-dependent. Maximal activation was observed at 37 C, but only partial activation took place at 4 C. A small amount of deoxycholate was required for Mg++ activation of deoxycholate-inactivated oxidase. Two pH optima were found for Mg++ activation of deoxycholate-inactivated oxidase, pH 5.3 and 7.3. Significant amounts of activation of the inactive oxidase occurred in the absence of Mg++ with an optimum at pH 5.0, with essentially no Mg++-independent activation demonstrable at pH 7.0.
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Selected References
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- Bishop D. G., Rutberg L., Samuelsson B. The solubilization of the cytoplasmic membrane of Bacillus subtilis by sodium dodecyl sulphate. Eur J Biochem. 1967 Nov;2(4):454–459. doi: 10.1111/j.1432-1033.1967.tb00159.x. [DOI] [PubMed] [Google Scholar]
- Broberg P. L., Smith L. The cytochrome system of Bacillus megaterium KM. The presence and some properties of two CO-binding cytochromes. Biochim Biophys Acta. 1967 May 9;131(3):479–489. doi: 10.1016/0005-2728(67)90007-2. [DOI] [PubMed] [Google Scholar]
- Eisenberg R. C., Yu L., Wolin M. J. Divalent cation activation of deoxycholate-solubilized and -inactivated membrane reduced nicotinamide adenine dinucleotide oxidase of Bacillus megaterium KM. J Bacteriol. 1970 Apr;102(1):172–177. doi: 10.1128/jb.102.1.172-177.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eisenberg R. C., Yu L., Wolin M. J. Masking of Bacillus megaterium KM membrane reduced nicotinamide adenine dinucleotide oxidase and solubilization studies. J Bacteriol. 1970 Apr;102(1):161–171. doi: 10.1128/jb.102.1.161-171.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROTHFIELD L., HORECKER B. L. THE ROLE OF CELL-WALL LIPID IN THE BIOSYNTHESIS OF BACTERIAL LIPOPOLYSACCHARIDE. Proc Natl Acad Sci U S A. 1964 Oct;52:939–946. doi: 10.1073/pnas.52.4.939. [DOI] [PMC free article] [PubMed] [Google Scholar]