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. 1970 Sep;103(3):560–568. doi: 10.1128/jb.103.3.560-568.1970

Asymmetric Template Function of Microbial Deoxyribonucleic Acids: Transcription of Ribosomal and Soluble Ribonucleic Acids

Lola Margulies 1, Virgilija Remeza 1, Rivka Rudner 1
PMCID: PMC248127  PMID: 4319834

Abstract

In Bacillus subtilis and Escherichia coli, 16 and 23S ribosomal ribonucleic acid (rRNA) hybridize exclusively with the heavy (H) strand of methylated albuminkieselguhr (MAK)-fractionated complementary deoxyribonucleic acid (DNA) strands. All the soluble RNA (4S RNA) in B. subtilis and 66 to 75% of the 4S RNA in E. coli also hybridize with the H strand. Interspecific hybridization shows that E. coli 23S rRNA also binds selectively to the DNA H strand of Salmonella typhimurium. The hybridization peak for all three cellular RNA components is specifically located in the late-eluting region of the absorbance profile of the DNA H strand. The early-eluting region of the light (L) strand preferentially inhibits the hybridization between the peak region of the H strand and 23S rRNA. These regions are considered to represent the transcribing sequences and their complements for 23S rRNA in the separated H and L strands of DNA, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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