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. 1970 Sep;103(3):650–655. doi: 10.1128/jb.103.3.650-655.1970

Regulation of Thymidine Metabolism in Escherichia coli K-12: Optimal Conditions for the Assay of 1,5-Phosphodeoxyribomutase in Ultrasonic Extracts

Robert J Bonney a,1, Herbert Weinfeld b
PMCID: PMC248139  PMID: 4919987

Abstract

Assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of Escherichia coli K-12. The enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1,6-diphosphate for optimal activity. When cells are grown in minimal medium with glycerol as the carbon source and supplemented with 10−3m thymidine, phosphodeoxyribomutase is induced three- to four-fold. Cell pellets may be frozen for 24 hr before sonic disruption without loss of enzyme activity. The assay is highly reproducible.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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