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. 1970 Sep;103(3):671–678. doi: 10.1128/jb.103.3.671-678.1970

Galactose Transport in Saccharomyces cerevisiae II. Characteristics of Galactose Uptake and Exchange in Galactokinaseless Cells

Shou-Chang Kou a,1, Michael S Christensen a, Vincent P Cirillo a,2
PMCID: PMC248142  PMID: 5474882

Abstract

The characteristics of the inducible galactose system in Saccharomyces cerevisiae were studied by using the nonmetabolized galactose analogues, l-arabinose and d-fucose, and galactokinaseless and transportless mutants. Induced wild-type cells transport l-arabinose by facilitated diffusion. Transportless cells transport neither galactose nor l-arabinose above the noninduced rate, whereas galactokinaseless cells transport galactose l-arabinose and d-fucose by facilitated diffusion. Determination of unidirectional rate of 14C-labeled galactose uptake by preloaded galactokinaseless cells, containing a large unlabeled free-galactose pool, showed that the rate of galactose uptake by facilitated diffusion is greater than the rate of galactose metabolism at similar external galactose concentrations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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